Article Sidebar

Submitted
Apr 22, 2021
Published
Apr 22, 2021
Download
PDF
Statistic
Read Counter : 34 Download : 23

Main Article Content

Abstract

OBJECTIVE


The aim of this study was to evaluate anti-arthritic activity of ethanolic extract of Chloroxylon swietenia leaves in Male Wister Albino rats using in-vitro and in-vivo methods.


METHODS


Animals were divided into five groups of six animals each as follows:


GROUP-I - Normal rats given vehicle alone 2% Tween 80, (p.o).


GROUP-II -Arthritic untreated rats.


GROUP-III -Arthritic rats treated with 10mg/kg (p.o) standard Diclofenac sodium.


GROUP-IV -Arthritic rats treated with 100mg/kg (p.o) of ethanolic extract.


GROUP-V –Arthritic rats treated with 200mg/kg (p.o) of ethanolic extract.


The inducing agent used was Complete Freund's adjuvant (CFA). The biochemical parameters like erythrocyte sedimentation rate (ESR), red blood cell (RBC), Hemoglobin (Hb), and total WBC count was observed which were the major markers of arthritis and uric acid analysis (plasma). A significant inhibition of paw edema volume and body weight was observed from day 0th, 7th, 14th, 21st and 28th day in the treated groups.


RESULTS


A significant increase in body weight, reduction in paw volume of both hind legs were observed in FCA induced arthritis rats. A decrease in levels of RBC and hemoglobin were observed in arthritic rats. There was a significant improvement in the levels of hemoglobin and RBC in Chloroxylon swietenia treated rats. The increased levels of WBC, ESR were significantly suppressed in the extract administered arthritic group.


CONCLUSION


It may be concluded that the ethanolic extract of Chloroxylon swietenia at two different concentrations (100mg/kg and 200mg/kg) possessed significant anti-arthritic activity as compared to standard drug.  

Keywords

Chloroxylon swietenia Freund’s adjuvant Diclofenac sodium

Article Details

How to Cite
Goverdhan Reddy Puchchakayala, Kumara Swamy Damerakonda, & Renuka. B. (2021). Evaluation of anti-arthritic activity of aqueous extract of leaves of chloroxylon swetenia in adjuvant induced arthritis in rats. International Journal of Research in Pharmacology & Pharmacotherapeutics, 4(3), 320-328. https://doi.org/10.61096/ijrpp.v4.iss3.2015.320-328
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. [1]. Ahlmen M, Svensson B, Albertsson K, influence of gender on assessments of disease activity and function in early rheumatoid arthritis in relation to radiographic joint damage. Ann Rheum Dis, 2010, 69(1): 230-233.
  2. [2]. Agarwal R.B, and Rangari V.D, “Phytochemical investigation and evaluation of anti-inflammatory and anti-arthritic activities of essential oil of Strobilanthus ixiocephala Benth,” Indian Journal of Experimental Biology, vol. 41,pp. 890–894,2003.
  3. [3]. Biswakanth K, Suresh Kumar RB, Indrajit K, Narayan D, Asis B, Upal KM, Pallab KH. Antioxidant and Invitro anti-inflammatory activities of Mimusops elengi leaves. APJTB.2012, S976-S980.
  4. [4]. Bing Lin , HongZhang , Xiang-XiangZhao , KhalidRahman , YingWang , Xue- Qin Ma, Cheng-Jian Zheng, Qiao-YanZhang, TingHan, Lu-PingQ in Inhibitory effects of the root extract of Litsea cubeba (lour.) pers. on adjuvant arthritis in rats Journal ofEthnopharmacology147(2013)327–334
  5. [5]. Begum VH, Sadique J, Long Term effect of herbal drug Withania somnifera on adjuvant induced arthritis on rats. Ind J Exp Biol1988; 26: 877-882.
  6. [6]. HochbergM.C, Arnold C.M, Hogans B.B and Spivak J.L, Serum immunoreactive erythropoietin in rheumatoid arthritis: impaired response to anemia, Arthritis Rheum. 31 (1988) 1318–1321.
  7. [7]. Jieyun Jiang , Qiang Xu Immunomodulatory activity of the aqueous extract from rhizome of Smilax glabra in the later phase of adjuvant-induced arthritis in rats. Journal of Ethnopharmacology 85 (2003) 53–59.
  8. [8]. Kamalesh kumar, Ganesh.M, Baskar.S, Srinivasan.k, Kanagasabai.R, Sambathkumar.R, Sureshkumar.S, and Sivakumar.T Evaluation of anti-inflammatory activity and toxicity studies of Chloroxylon sweitenia Vol : XXV (3&4) ,2006, page 33-43.
  9. [9]. Maria M, Engeniusz M, Miroslaw K, Maria K, Iwona P. Adjuvant induced disease in rats. Clinical findings and morphological and biochemical changes in blood and histological changes in internal organs, Rheumatologia 21 (1983) 213–245.
  10. [10]. Marcelletti J.F, Nakamura R.M, Assessment of serological markers associated with rheumatoid arthritis. Diagnostic autoantibodies and conventional disease activitymarkers, Clin. Appl. Immunol.Rev. 4 (2003) 109–123.
  11. [11]. Medicinal Plants and Traditional Medicine in Africa. Sofowora, A [M]. New York: John Willey and Sons, 1982, 142.
  12. [12]. Mowat A.G, Anaemia in rheumatoid arthritis, Mod. Trends. Rheumatol. 2 (1971) 106–116
  13. [13]. Nilip Kanti Deb, Gouri Kumar Dash. A Review on Ethnopharmacolgy, Phytochemistry and Bioactivity of Chloroxylon swietenia DC International Journal of Emerging Trends in Pharmaceutical Sciences ISSN 2320 – 8783, Volume 1, Issue 1, February 2013.
  14. [14]. Panoulas V.F, Milionis H.J, Douglas K.M.J, Nightingale P, Kita M.D, Klocke R, Elisaf M.S, and Kitas G.D Association of serum uric acid with cardiovascular disease in rheumatoid arthritis Rheumatology 2007;46:1466–1470
  15. [15]. Prashant Soni, Madan Singh Neeraj Upmanyu and Yogesh Shivhare In-vitro Anti-arthritic Activity of Manilkara zapota Linn; Asian J. Pharm. Tech. 1(4): Oct. - Dec. 2011; Page 123-124.
  16. [16]. Rajendran Mythiliypriya, Palanivelu Shanthi, Panchanadam Sachdanandam Salubrious effect on Kalpaamrutha, a modified indigenous preparation in adjuvant induced arthritis in rats –A biochemical approach Chemico- Bilogical Interactions 173(2008)148-158.
  17. [17]. Sangeetha M, kousalya K, Lavanya R, Sowmya C, Chamundeshewari D, Umamaheshwari CR. Invitro anti-inflammatory and anti-arthritic activity of leaves of cleodendron inerme, research j of pharmaceutical, biological chem sciences 2011, (2):822-827.
  18. [18]. Umapathy E, Ndebia EJ, Meeme A, Adam B, Menziwa P, Nkeh-Chungag BN. AN experimental evaluation of Albuca setosa aqueous extract of on membrane stabilization, protein denaturation and white blood cell migration during acute inflammation. J Med plants research 2010, (4):789-795.
  19. [19]. Neeraj Upmanyu, Prashant Soni, Madan Singh and Yogesh Shivhare In-vitro Anti-arthritic Activity of Manilkara zapota Linn; Asian J. Pharm. Tech. 1(4): Oct. - Dec. 2011; Page 123-124.
  20. [20]. Pearson CM. Development of arthritis, periarthritis and periostitis in rats given adjuvants. Proc. Soc Exp Biol Med.1956; 91:95-101.