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Abstract

Sapota is one of the prominent fruits and belongs to family sapotaceae. India is the largest producer of sapota. In this present study, phytochemicals were analysed and antioxidant activity was determined for sapota fruit. The antioxidant capacity of the Manilkara zapota  L fruit extract was evaluated by three different in vitro methods: DPPH activity, hydrogen  peroxide scavenging activity and total antioxidant activity. The ethanolic extract showed best antioxidant activity almost equal to that of standard ascorbic acid. The antioxidant capacity of ethanolic extract may be due to its high phytochemical constituents. The high antioxidant capacity observed in aqueous and ethanolic extracts suggested that this plant could be used as an additive in the food industry providing good protection against oxidative damage. Results indicate that nutrient composition, phytochemical and antioxidant activity of sapota was promising. It is also a good source of antioxidants. These antioxidants are important in all of our diets, especially in infant diets. Thus sapota fruit can be used as a potent therapeutic agent.

Keywords

Antioxidant Capacity Manilkara zapota DPPH In Vitro Methods

Article Details

How to Cite
Agnes Jenitha X, & Bhuvaneshwari M. (2021). In vitro studies on phytochemical analysis and antioxidant activity of Manilkara zapota. International Journal of Research in Pharmacology & Pharmacotherapeutics, 5(2), 153-161. https://doi.org/10.61096/ijrpp.v5.iss2.2016.153-161

References

  1. [1]. Rampriya R, Vasantha Devi. K. P, Thahira Banu. A, (2015), Nutrient Content, Phytonutrient Composition and Antioxidant Activity of Sapota Pulp Powder, international journal of scientific research Volume: 4 Issue: 9 Sept 2015.
  2. [2]. Poornima. K, Cariappa M, Asha K, Kedilaya H.P and Nandini M, (2003), oxidant and antioxidant status in vegetarians and fish eaters. Indian journal of biochemistry, 18 (2) 197-205
  3. [3]. Shanmugapriya K, Saravan P.S, Harshapayal, Peer Mohammed S, Williams Binnie, (2011), antioxidant activity, total phenolic and flavonoid contents of artocarpus heterophyllus and Manilkara zapota seeds and its reduction potential International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491 Vol 3, Suppl 5.
  4. [4]. Chanda S. V and Nagani K. V, (2010), Antioxidant Capacity of Manilkara zapota L. Leaves Extracts Evaluated by Four in vitro Methods. Nature and Science 8 (10).
  5. [5]. Markpercival DR, (1998), Antioxidants. Clinical nutrition insights. NUT031 1/96 Rev. 10/98.
  6. [6]. Shimada, K., Fujikawa, K., Yahara, K., & Nakamura, T. Antioxidant properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of agricultural and food chemistry, 1992; 40: 945-948.
  7. [7]. Trease G.E. And Evans W.C a Textbook of pharmacogency, 1 4th Ed. Bailliere Tindall Ltd, London, 2013; 832.
  8. [8]. Prieto, P., M. Pineda and Aguilar, M. Spectrophotometric Quantitation of antioxidant capacity through the formation of phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Biochem, 1999; 269: 337-341.
  9. [9]. Klein SM, Cohen G, Cederaum AI. Production of formaldehyde during metabolism of dimethyl sulphoxide by hydroxyl radical generating system. Biochemistry 1991; 20: 6006-6012.
  10. [10]. Nabavi S. M, Ebrahimzadeh M. A, Nabavi S. F, Fazelian M, Eslami B, (2009) Pharmacognosy Magazine, 4,123-127.
  11. [11]. Ebrahimzadeh M. A, Pourmorad F, Hafezi S; Turk. Biol J, (2008), 32, 43-49.
  12. [12]. Dehpour A. A, Ebrahimzadeh M. A, Nabavi S. F, Nabavi S. M; Grasas Aceites, (2009), 60, 405-412.
  13. [13]. Nunes PX, Silva SF, Guedes RJ, Almeida S: Biological oxidations and antioxidant activity of natural products, Phytochemicals as nutraceuticals - Global Approaches to Their Role in Nutrition and Health. 2012
  14. [14]. Krishnaiah D, Sarbatly R, Nithyanandam RR: A review of the antioxidant potential of medicinal plant species. Food Bioprod Process. 2011, 89: 217-233.
  15. [15]. Gulcin I, Berashvili D, Gepdiremen A (2005): Antiradical and antioxidant activity of total anthocyanins from Perilla pankinensis decne. J Ethnopharmacol. 2005, 101: 287-293. 10.1016/j.jep.2005.05.006.
  16. [16]. Sahreen S, Khan MR, Khan RA, (2011): Phenolic compounds and antioxidant activities of Rumex hastatus D. Don. Leaves. J Med Plants Res. 2011, 5: 2755-2765.
  17. [17]. Prale milind and preethi, (2015), chickoo: a wonderful gift from nature, Ayurveda pharm, received on 14. 4.15.
  18. [18]. Mathangi T, prabhakaran p. Screening for antimicrobial activity of the leaf extracts of Marsilea quadrifolia against various bacterial pathogens. Herbal Tech Industry, 2012; 9 (8): 11-12.
  19. [19]. Trease GE and Evans WC, a textbook of Pharmacognecy. 14th ed.Bailliere Tindall Ltd., London, 2013; 832.