Evaluation of anti-oxidant and anxiolytic effects of ethanolic root extract of Cymbopogon flexousus in swiss albino mice

Abstract

The study aimed to evaluate the antioxidant and anxiolytic potential of the ethanolic root extract of Cymbopogon flexuosus (lemongrass) in Swiss albino mice. Antioxidant properties were assessed using in vitro assays, including DPPH radical scavenging and reducing power activities, which demonstrated significant free radical neutralization by the extract. For the anxiolytic evaluation, behavioral studies such as the Elevated Plus Maze (EPM), Open Field Test (OFT), and Light-Dark Box Test were conducted on Swiss albino mice after oral administration of varying doses of the extract (100, 200, and 400 mg/kg). The results indicated a dose-dependent reduction in anxiety-like behaviors, comparable to standard anxiolytic drug diazepam. Furthermore, the extract exhibited no observable toxic effects in acute toxicity studies, confirming its safety profile. The presence of bioactive compounds such as flavonoids, phenols, and terpenoids in the extract likely contributed to its antioxidant and anxiolytic activities. These findings suggest that Cymbopogon flexuosus root extract has the potential to mitigate oxidative stress and anxiety, providing a natural therapeutic alternative for managing anxiety-related disorders. Further investigations, including mechanistic studies and clinical trials, are recommended to validate its efficacy and pharmacological properties.