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The existing study was planned to evaluate the free radical scavenging activity of Digera muricata solvent extracts by different in vitro assays and compare it with total phenolic content. Digera muricata is a wild edible herb used traditionally for treating diabetes, urinary discharges, as laxative and to increase lactation after child birth. Different solvent extracts (petroleum ether, benzene, chloroform, methanol and aqueous) were assessed for their ability to scavenge free radicals such as DPPH, superoxide, and hydroxyl radicals, as well as their metal chelating capacity, reducing power along with  quantification  of phenolic content. All the solvents exhibited substantial free radical scavenging activity in a concentration dependent manner. Among the five extracts, the methanolic extract showed the highest free radical scavenging activity and yielded 32.38 mg/g gallic acid equivalents (GAE) of phenolic content. The study found a significant positive correlation between the total phenolic content and the antioxidant assays, indicating that phenolic compounds played a role in the antioxidant activity of D. muricata extracts. These findings suggest that the methanolic extract of D. muricata contains a significant amount of phenolic compounds, which could be accountable for its antioxidant activity. These results highlight the potential of D. muricata as a source of natural antioxidants and support its traditional use for various health conditions.


Free radicals, Digera muricata, antioxidant, plant extracts, phenolics, DPPH

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How to Cite
S. Saini. (2023). Estimation of total phenolic content and antioxidant activity of digera muricata extracts by various in vitro assays. International Journal of Research in Pharmacology & Pharmacotherapeutics, 12(2), 139-145.


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