Main Article Content
Abstract
The folk medicine system utilizes plant products for the treatment of numerous diseases. Studies by various researchers have proven that plants are major sources of drugs. The curative properties of medicinal plants are due to the presence of various chemical substances of different compositions, which occur as secondary metabolites. Leaves of Pisonia grandis were extracted using various solvents with a Soxhlet apparatus to determine the structural formulas of various compounds isolated from Pisonia grandis and conduct phytochemical screening of crude drug extracts. The present work revealed that the phytochemical analysis of the ethanolic extract of Pisonia grandis contains carbohydrates, proteins, flavonoids, saponins, tannins, steroids, and glycosides. The chloroform extract shows the presence of carbohydrates, proteins, amino acids, alkaloids, tannins, steroids, and glycosides. The benzene extract indicates the presence of tannins, terpenoids, gums, carbohydrates, proteins, steroids, and glycosides. The moisture content in dry leaves of Pisonia grandis is 4.7%, and the total ash value is 12.6%. Physicochemical parameters such as ash value and moisture loss determination were used to assess the quality and purity of the crude drug. The biochemical analysis of Pisonia grandis revealed the presence of carbohydrates, proteins, and other bioactive compounds. Pisonia grandis possesses considerable levels of bioactive compounds and, therefore, can be used as a potential source of drugs. The plant has significant potential to cure fresh wounds, ulcers, inflammations, and skin complaints.
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References
- 1. Alhassan AJ, Sule MS, Atiku MK, Wudil AM, Abubakar H, Mohammed SA. Effects of aqueous avocado pear (Persea americana) seed extract on alloxan-induced diabetes rats. Greener J Med Sci. 2012;2(1):5-11.
- 2. Anitha VT, Antonisamy JM, Jeeva S. Antibacterial studies on PG (Blume) HG. Hallier and Elephantopus scaber L. Asian Pac J Trop Med. 2012;5(1):52-57.
- 3. Annapoorna MK, Sudheesh PT, Lakshmanan L, Lakshmi R. Biochemical properties of Hemigraphis alternata incorporated chitosan hydrogel scaffold. Carbohydr Polym. 2013;92(2):1561-1565.
- 4. Arun KK, Nimmanapalli PR, Chaitanya RK, Roy K. Ethyl acetate extract of PG leaves shows anti-inflammatory and wound healing properties and inhibits 5-lipoxygenase and cyclooxygenase-1 and -2 enzymes. J Med Plants Res. 2013;7(37):2783-2791.
- 5. Asha G, Nishath TK, Benny PJ. Evaluation of phytochemical analysis, anti-oxidant, and anti-elastase activity of Hemigraphis colorata. Int J Pharmacogn Phytochem Res. 2014;5(4):292-298.
- 6. Bhardwaj S, Deepika G. Study of acute, sub-acute, and chronic toxicity tests. Int J Adv Res Pharm Bio Sci. 2012;2(2):103-129.
- 7. Biju CR, Nimmi M, Byju K, Arunlal VB. A review on PG (Blume). Int J Innov Pharm Sci Res. 2015;3(7):32-40.
- 8. Daniel AD, Sylvia U, Ute R. A historical overview of natural products in drug discovery. Metabolites. 2012;2:303-336.
- 9. Devi PM. Review on pharmacological activity of PG (Blume) H.G. Hallier. Int J Herbal Med. 2013;1(3):120-121.
- 10. Eleanor R, Keith W, Hilary M. The detection, identification, and structural elucidation of unknown contaminants during ToF screening for pesticides in river water using an integrated software approach. Waters. 2014:1-13.
- 11. Gayathri V, Lekshmi P, Kala DR, Padmanabhan RN. Antidiabetic activity of PG [whole plant ethanol extract] in streptozotocin-induced diabetic rats and toxicity evaluation of the extract. Int J Pharm Pharm Sci. 2012;4(2):475-480.
- 12. Karpagam N, Naveen Prasad R, Vasanthi N. Antimicrobial and phytochemical screening of Solanum torvum. Herbal Tech Indus. 2008:20-45.
- 13. Kokate CK, Purohit AP, Gokhale SB. Textbook of Pharmacognosy. 3rd ed. Pune: Nirali Prakashan; 2001.
- 14. Lim HK, Anees AA, Alkarkhi FM, Sufia H. Extraction, separation, and identification of chemical ingredients of Elephantopus scaber L. using factorial design of experiment. Int J Chem. 2009;1(1):36-49.
- 15. Mukherjee PK, Wahile A. Integrated approach towards drug development from Ayurveda and other Indian systems of medicine. J Ethnopharmacol. 2006;103(1):25-35.
- 16. Ramnivas Rangheetha, Malaiswamy Suganya, Krishnan Sridharan, Muthusamy Sureshkumar, Govindasami Vivekanandhan, Manokaran, et al. Evolution of phytochemical constituents of PG (Burm.f.) T. Anderson leaf extract. 2016;8(6):335-338.
- 17. Sadasivam S, Manikam A. New Age International (P) Ltd., Publisher, New Delhi; 2008.
- 18. Saravanan J, Shariff WR, Joshi Narasimhachar H, Vartharajan R, Joshi VG, Karigar AA. Preliminary pharmacognostical and phytochemical studies of leaves of Hemigraphis colorata. Res J Pharmacogn Phytochem. 2010;2(1):15-17.
- 19. Subramoniam A, Evans DA, Rajasekharan S, Sreekandan Nair G. Effect of PG (Blume) H. G. Hallier leaf on wound healing and inflammation in mice. 2001;33:283-285.
- 20. T. T. Akhil, Punieethaprabhu. Evaluation of anti-oxidant, anti-inflammatory, and cytotoxicity potential of PG Int J Pharm Sci Res. 2013;4(9):3477-3483.
References
1. Alhassan AJ, Sule MS, Atiku MK, Wudil AM, Abubakar H, Mohammed SA. Effects of aqueous avocado pear (Persea americana) seed extract on alloxan-induced diabetes rats. Greener J Med Sci. 2012;2(1):5-11.
2. Anitha VT, Antonisamy JM, Jeeva S. Antibacterial studies on PG (Blume) HG. Hallier and Elephantopus scaber L. Asian Pac J Trop Med. 2012;5(1):52-57.
3. Annapoorna MK, Sudheesh PT, Lakshmanan L, Lakshmi R. Biochemical properties of Hemigraphis alternata incorporated chitosan hydrogel scaffold. Carbohydr Polym. 2013;92(2):1561-1565.
4. Arun KK, Nimmanapalli PR, Chaitanya RK, Roy K. Ethyl acetate extract of PG leaves shows anti-inflammatory and wound healing properties and inhibits 5-lipoxygenase and cyclooxygenase-1 and -2 enzymes. J Med Plants Res. 2013;7(37):2783-2791.
5. Asha G, Nishath TK, Benny PJ. Evaluation of phytochemical analysis, anti-oxidant, and anti-elastase activity of Hemigraphis colorata. Int J Pharmacogn Phytochem Res. 2014;5(4):292-298.
6. Bhardwaj S, Deepika G. Study of acute, sub-acute, and chronic toxicity tests. Int J Adv Res Pharm Bio Sci. 2012;2(2):103-129.
7. Biju CR, Nimmi M, Byju K, Arunlal VB. A review on PG (Blume). Int J Innov Pharm Sci Res. 2015;3(7):32-40.
8. Daniel AD, Sylvia U, Ute R. A historical overview of natural products in drug discovery. Metabolites. 2012;2:303-336.
9. Devi PM. Review on pharmacological activity of PG (Blume) H.G. Hallier. Int J Herbal Med. 2013;1(3):120-121.
10. Eleanor R, Keith W, Hilary M. The detection, identification, and structural elucidation of unknown contaminants during ToF screening for pesticides in river water using an integrated software approach. Waters. 2014:1-13.
11. Gayathri V, Lekshmi P, Kala DR, Padmanabhan RN. Antidiabetic activity of PG [whole plant ethanol extract] in streptozotocin-induced diabetic rats and toxicity evaluation of the extract. Int J Pharm Pharm Sci. 2012;4(2):475-480.
12. Karpagam N, Naveen Prasad R, Vasanthi N. Antimicrobial and phytochemical screening of Solanum torvum. Herbal Tech Indus. 2008:20-45.
13. Kokate CK, Purohit AP, Gokhale SB. Textbook of Pharmacognosy. 3rd ed. Pune: Nirali Prakashan; 2001.
14. Lim HK, Anees AA, Alkarkhi FM, Sufia H. Extraction, separation, and identification of chemical ingredients of Elephantopus scaber L. using factorial design of experiment. Int J Chem. 2009;1(1):36-49.
15. Mukherjee PK, Wahile A. Integrated approach towards drug development from Ayurveda and other Indian systems of medicine. J Ethnopharmacol. 2006;103(1):25-35.
16. Ramnivas Rangheetha, Malaiswamy Suganya, Krishnan Sridharan, Muthusamy Sureshkumar, Govindasami Vivekanandhan, Manokaran, et al. Evolution of phytochemical constituents of PG (Burm.f.) T. Anderson leaf extract. 2016;8(6):335-338.
17. Sadasivam S, Manikam A. New Age International (P) Ltd., Publisher, New Delhi; 2008.
18. Saravanan J, Shariff WR, Joshi Narasimhachar H, Vartharajan R, Joshi VG, Karigar AA. Preliminary pharmacognostical and phytochemical studies of leaves of Hemigraphis colorata. Res J Pharmacogn Phytochem. 2010;2(1):15-17.
19. Subramoniam A, Evans DA, Rajasekharan S, Sreekandan Nair G. Effect of PG (Blume) H. G. Hallier leaf on wound healing and inflammation in mice. 2001;33:283-285.
20. T. T. Akhil, Punieethaprabhu. Evaluation of anti-oxidant, anti-inflammatory, and cytotoxicity potential of PG Int J Pharm Sci Res. 2013;4(9):3477-3483.