Article Sidebar

Submitted
Apr 7, 2023
Published
Dec 19, 2020
Download
PDF
Statistic
Read Counter : 17 Download : 15

Main Article Content

Abstract

Today Unani system of treatment practised, taught and researched under its local names in over 20 countries including Afghanistan, China, Canada, Denmark, Germany, Finland, Netherlands, Norway, Poland, Korea, Japan, Saudi Arabia, Sweden, Switzerland, Turkey, UK and USA. Unani system of medicine is unmatched in treating chronic diseases like arthritis, asthma, mental, cardiac and digestive disorders, urinary infections, and sexual diseases. Unani medicine established that disease was a natural process and that symptoms were the reactions of the body to the disease. Unani medicine plays a vital role when the individual experiences the humoral imbalance. Plan of study there is a  need to establish internationally recognized methodology for quality standardization of traditional herbal medicines like multiple marker based quantification, determination of total metabolite content or application of hyphenated techniques such as LC-PDA, LC-MS or GC-MS which will give better understanding of bioactive multi component herbal formulations PLAN OF WORK Selection of formulations, Physico-chemical evaluation of formulations, Determination of contaminants, Chemical contaminants (Heavy metal, Pesticides residues), Fungal contaminants (Aflatoxins), Development of HPTLC fingerprinting, Development of fingerprint profile by GC-MS, Development of analytical methods for the quantification of marker Validation of the developed method, MATERIALS USED FOR THE STUDY Since the work carried out in present investigation was the part of an AYUSH/Govt. of India sponsored project for the development of analytical standards of some compound Unani formulations to be included in Unani Pharmacopeia/Formulary. Unani formulations were selected for analysis for General physico-chemical evaluations like organoleptic characteristics, loss on drying at 105° C, moisture content by Karl Fischer method, total ash, acid insoluble ash, water soluble ash, pH of 1 % and 10 % suspensions, petroleum ether extractive value, chloroform extractive value, acetone extractive value, methanol extractive value , total phenolic content by UV, content of sugar by Anthrone reagent method, aflatoxins (HPLC) and pesticide (GC-MS) showed absence of contaminants/ or below the acceptable limit in all batches of Itrifal-e- Badiyan, HPTLC fingerprinting of methanol, chloroform and petroleum ether extracts of Itrifal- e-Badiyan showed the presence of five, nine and nine peaks in chromatograms after visualizing with anisaldehyde sulphuric acid. A simple rapid and economic simultaneous HPLC method was developed and validated for the quantification of gallic acid, ellagic acid and ascorbic acid, in in Itrifal-e-Badiyan formulation.

Keywords

HPLC, Badiyan, Unani, physicochemical evaluation, HPTLC fingerprints

Article Details

How to Cite
Mohammed Minhajuddin. (2020). New Advanced Analytical Methodologies Developed for Quality Control of Herbal Extracts of Foeniculum Vulgare Mill Formulations. International Journal of Research in Pharmacology & Pharmacotherapeutics, 9(4), 279-286. https://doi.org/10.61096/ijrpp.v9.iss4.2020.279-286
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. 1. Ahmad, B., Akhtar, J., 2007. Review article Unani system of medicine. Pharmacogn. Rev. 1, 210-214.
  2. 2. Ahmad, S., Rizwan, M., Parveen, R., Mujeeb, M., Aquil, M. 2008. A validated stability-indicating TLC method for determination of forskolin in crude drug and pharmaceutical dosage form. Chromatographia 67, 441-447.
  3. 3. Alam, P., Ali, M., Ahmad, S. 2009. A validated HPLC method for estimation of cordifolioside A in Tinospora cardifolia, Miers and marketed formulations. J. Chrom. Sci. 47, 910-913.
  4. 4. Amorati, R., Pedulli, G.F., Valgimigli, L., 2011. Kinetic and thermodynamic aspects of the chain-breaking antioxidant activity of ascorbic acid derivatives in non-aqueous media. Org. Biomol. Chem. 9, 3792-3800.
  5. 5. Anand, S., Muthusamy, V.S., Sujatha, S., Sangeetha, K.N., Bharathi, R.R., Sudhagar, S., Poornima, D.N., Lakshmi, B.S., 2010. Aloe emodin glycosides stimulates glucose transport and glycogen storage through PI3K dependent mechanism in L6 myotubes and inhibits adipocyte differentiation in 3T3L1 adipocytes. FEBS Lett. 16, 3170-3178.
  6. 6. Anonymous, 1996. Indian Pharmacopoeia, Vol-II, Ministry of Health and Family Welfare, Government of India, New Delhi, pp. 112.
  7. 7. Ansari, M.J., Ahmad, S., Kohli, K., Ali, J., Khar, R.K. 2005. Stability- indicating HPTLC determination of curcumin in bulk drug and pharmaceutical formulations. J. Pharm. Biomed. Anal. 39, 132-138.
  8. 8. Aoki, F., Nakagawa, K., Tanaka. A., Matsuzaki, K., Arai, N., Mae, T., 2005. Determination of glabridin in human plasma by solid-phase extraction and LC-MS/MS. J. Chromatogr. B. 15, 828, 70-74.
  9. 9. Bajpai, M., Pande, A., Tewari, S.K., Prakash, D., 2005. Phenolic contents and antioxidant activity of some food and medicinal plants. Int. J. Food Sci. Nutr. 56, 287-291.
  10. 10. Gerbeth, K., Meins, J., Kirste, S., Momm, F., Schubert, Z.M., Abdel, T.M., 2011. Determination of major boswellic acids in plasma by high-pressure liquid chromatography/mass spectrometry. J. Pharm. Biomed. Anal. 15, 998- 1005.
  11. 11. Imark, C., Kneubuehl, M., Bodmer, S., 2001. Occurrence and activity of natural antioxidants in herbal spirits. Innovat. Food Sci. Emerg. Tech. 1, 239- 242.
  12. 12. Kamal, Y.T., Singh, M., Tamboli, E.T., Parveen, R., Ahmad, S., 2012b. Reverse -Phase Gradient High Performance Liquid Chromatography Method for the Determination of Glabridin in Crude Drug and Polyherbal Formulations, J. of Chrom. Sci. DOI:10.1093/chromsci/bms063.
  13. 13. Lalla, J.K., Hamrapurkar, P.D., Mamania, H.M., 2000. Application of HPTLC to alternative medicines, qualitative and quantitative evaluation of the Ayurvedic formulation ‘Triphala Churna’. J. Planar Chromatogr. 13, 390-394.
  14. 14. Lee, S., Khoo, C., Halstead, C.W., Huynh, T., Bensoussan, A., 2007. Liquid chromatographic determination of 6-, 8-, 10-gingerol and 6-shogaol in ginger (Zingiber officinale) as the raw herb and dried aqueous extract. J. AOAC Int. 5, 1219-1226.
  15. 15. Park, B.S., Son, D.J., Choi, W.S., Takeoka, G.R., Han, S.O., Kim, T.W., Lee, S.E., 2008. Antiplatelet activities of newly synthesized derivatives of piperlongumine. Phytother. Res. 22, 1195-1199.
  16. 16. Qian, Z.M., Wan, J.B., Zhang, Q.W., Li, S.P., 2008. Simultaneous determination of nucleobases, nucleosides and saponins in Panax notoginseng using multiple columns high performance liquid chromatography. J. Pharm. Biomed. Anal. 48, 1361-1367.
  17. 17. Rates, S.M.K., 2001. Plants as source of drugs. Toxicon, 39, 603–613.
  18. 18. Reich, E., Anne, S., Widmer V., Jorns, R., Wolfram, E., Debatt, A., 2006. HPTLC of flavanoids. Camag Bibliographic service, 99.
  19. 19. Szapary, P.O., Wolfe, M.L., Bloedon, L.T., Cucchiara, A.J., DerMarderosian, A.H., Cirigliano, M.D., Rader, D.J., 2003. Guggulipid for the treatment of hypercholesterolemia: a randomized controlled trial. JAMA. 13, 765-772.
  20. 20. Verma, J.K., Joshi, A.V., 2007. Rapid HPTLC method for identification and quantification of curcumin, piperine and thymol in an Ayurvedic formulation. J. Planar. Chromatogr. 19, 398-400.