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Pharmaceutical excipients such as cellulose (CL) and cyclodextrin (CD) derivatives are chiral in nature. Currently, these derivatives are also used as chiral stationary phases (CSPs) in liquid chromatography. The  stereoselectivity of these derivatives towards enantiomers that may modulates the release of chiral drugs. Based on this, it has been hypothesized that, the stereoselectivity of the enantiomers with the CL and CD excipients may alter the release of chiral drug. Hence, the present study aimed to investigate the influence of CL and CDs on stereoselective release of formulation containing racemic ondansetron (rac-OND). In this study, formulation containing rac-OND and selected CLs; carboxymethylcellulose (CMC), hydroxypropyl methylcellulose (HPMC), and microcrystalline cellulose (MC) and CDs; beta-cyclodextrin (β-CD) and hydroxypropyl β-cyclodextrin (HCD) was prepared. Subsequently, these formulations were evaluated for enantioselective release by HPLC method. The results revealed that the formulations containing microcrystalline cellulose and hydroxypropyl methylcellulose showed slightly different release of the two enantiomers at the end of the dissolution profile. In addition, the effect of enantioselective interaction was investigated by changing content of chiral excipient and pH (1.5, 4.6 and 7.4) to support the enantioselectivity obtained on dissolution.


Ondansetron, Pharmaceutical excipients, Stereoselectivity, Liquid chromatography

Article Details

How to Cite
K. Selvakumar, & J. Joysa Ruby. (2022). Evaluation of stereoselective dissolution of racemic ondansetron by hplc method. International Journal of Research in Pharmacology & Pharmacotherapeutics, 11(4), 203-209. Retrieved from


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