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Bauhinia purpurea is a moderate sized ornamental evergreen tree belongs to the family caesalpinioideae. Kathkors & Gondas tribes of India used young pods, mature seeds are used to treat ulcer, glandular swelling and stomach tumor as  laxative, anthelmintic, piles & blood dysentery, diarrhea, goiter, carminative, gripping pain from stomach and bowel,  astringent, anti-obesity, respiratory disorder, mensuration trouble, fibrolytic, lymphadenitis, asthma, cyto-toxic,  anti-malarial, cytotoxic and leucorrhoea. Phytochemical review showed the presence of glycosides, phenolic compounds, saponins, flavonoids, tri terpenoids, fatty acids and phyto sterols. Literature review revealed the pharmacognostical preliminary phytochemical evaluation was not investigated. An attempt was taken to study the above including estimation and in vitro anti-oxidant activity. It bears compound leaf with reticulate venation, glabourous surface, orbiculate shape and entire margin.  Microscopy of leaves showed the presence of collenchyma, cortex, epidermis, parenchymatous cells, pericyclic fibres, stomata, xylem and phloem region in transverse section of petiole, midrib and lamina. Powder microscopy showed reticulate vessels, palisade cells, fibre bundles, prismatic crystals. The quantitative microscopical parameters such as epidermal number (upper epidermis=2432-2720/mm2, lower epidermis= 1790-2880/mm2), vein islet (43-56/mm2), vein termination number (11-15/mm2), stomatal index (19/mm2) and stomatal number (432-560/mm2) was determined. Physicochemical parameters such as loss of drying (5.04±0.299%w/w), aqueous extractive value (36.80±0.679%w/w), ethanol extractive value (22.67±0.715%w/w), total ash (6.2±0.04%w/w) was determined. Phytochemical screening indicated the presence of phenols, sterols, tannins, aminoacids, carbohydrates and glycosides. Quantitative estimation of gallic acid (37mg/gm), tannic acid (120mg/gm) & rutin (547mg/gm) were determined and correlated with standard. Invitro anti-oxidant activities such as hydrogen peroxide (6.72µg/mL), nitric oxide (7.39 µg/mL) and total anti-oxidant (16.92µg/mL) method were evaluated in comparison with ascorbic acid used as standard.


Bauhinia purpurea phytochemical investigation antioxidant

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A.Krishnaveni, D. Kothai Andal, P. G. S. Danyalakshmi, T. Venkata Rathina Kumar, & A. Abdul Hasan Sathali. (2022). Pharmacognostical, preliminary phytochemical evaluation, estimation of phytoconstituents and in-vitro anti-oxidant activity of Bauhinia purpurea linn. International Journal of Research in Pharmacology & Pharmacotherapeutics, 11(2), 47-61.


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