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Adenanthera pavonina L(mimosidae) is a deciduous tree, also known as red sandal wood or coral wood tree. The leaves and bark of this plant are used by Irula tribals for curing various ailments like diarrhoea, asthma, jaundice, tuberculosis, leprosy, rheumatism, hypertension, wound healing, diabetes, inflammation, and cancer. Leaves posseses anti-inflammatory, anti- arthritic, antioxidant, antinociceptive, antiemetic, hypoglycemic, antibacterial, cytotoxicity, radical scavenging activity. Phytochemical review showed the presence of carbohydrates, proteins, flavonoids, phenols, sterols, saponins, glycosides, tannins, alkaloids. The literature review revealed the pharamacognostical preliminary phytochemical evaluation was not investigated. An endeavor study was taken to investigate pharmacognostical, preliminary phytochemical screening, quantitative estimation and invitro anti-oxidant activity. Transverse section of petiole, rachis, midrib, the stomata are strictly paracytic type, thick walled uniserate, simple trichomes are present. Powder microscopy of leaf showed presence of thick walled simple trichomes, reticulate vessels, prismatic crystals and scelreids. The quantitative microscopical parameters such as stomatal number (100-128/mm2), stomatal index (11-13/mm2), vein islet number (2-3/mm2) and vein termination number(51-64/mm2), palisade ratio(3-5/mm2). Physicochemical parameters such as loss on drying (6.61±0.39%w/w), total ash(15.83± 0.05%w/w), ethanol extractive value(26.30±0.57),aqueous extractive value(36.73±2.05). Preliminary phytochemical screening of this plant revealed the presence of carbohydrates, proteins, tannins, flavonoid, steroids, saponins, phenols. Quantitative estimation of gallic acid (35gm/gm), tannic acid (86gm/gm) & rutin (252mg/gm) were determined and correlated with standard. In-vitro anti-oxidant by hydrogen peroxide (16.32 µg/mL), nitric oxide (5.58 µg/mL), total antioxidant(24.67 µg/mL) & method were estimated and in comparison with ascorbic acid used as standard.


Adenanthera pavonina phytochemical quantitative estimation antioxidant

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A. Krishnaveni, P. G. S. Danyalakshmi, D. Kothai Andal, T. Venkata Rathina Kumar, & A. Abdul Hasan Sathali. (2022). Pharmacognostical, preliminary phytochemical screening, estimation of phyto constituents and its in-vitro antioxidant activity of Adenanthera pavonina linn (leaves). International Journal of Research in Pharmacology & Pharmacotherapeutics, 11(2), 62-75. Retrieved from


  1. 1. Gamble JS. Flora of madras. Vol I, Botanical survey of India. Calcutta, PP. 286-9.
  2. 2. Nadkarni. The Indian materia medica. [Vol I, London J& A Churchill ltd]. 1959;104 gloucester place, w.1:182.
  3. 3. Chatterjee A, Dr. Pakrashi SC. The Treatise on Indian Medicinal plants. Vol. II. publications & information directorate. New Delhi: Council of Scientific and Industrial Research, 1992, PP. 16-21.
  4. 4. Varier PS. Indian medicinal plant-Vol. I. Orientlongman Ltd Frist publication, 1994, PP. 58-61.
  5. 5. Dissanayake RW, Jayantha R. Hypoglycemic and antihyperglycaemic Activities of an Aqueous Leaf Extract of Adenanthera pavonina (Fabaceae) in Rats. International Journal of Pharmaceutical Research andAllied Sciences. 2016;5:34-9.
  6. 6. Dhivya S, Kalaichelvi K. Ethno medicinal knowledge of plants used by Irula tribes, nellithurai beat,the Nilgiris, Tamil Nadu, India. Asian J Med Sci. 2016;7(5):124-8. doi: 10.3126/ajms.v7i5.14822.
  7. 7. Ghani A. Medicinal Plants of Bangladesh (Chemical Constituents and uses). 2nd ed. Dhaka: Asiatic Society of Bangladesh-1000. Bangladesh; 2003. p. 331-2.
  8. 8. Sofowara A. Screening plants for bioactive agents. In: Medicinal plants and traditional medicine in Africa. 2nd ed. Spectrum Books Ltd. Ibadan, Nigeria: Sunshine House; 1993. p. 134-56.
  9. 9. Mayuren C, Ilavarasan R. Anti-inflammatory activity of ethanolic leaf extracts from Adenanthera pavonina (L) in Rats. J Young Pharmacists. 2009;1(2):125-8. doi: 10.4103/0975-1483.55744.
  10. 10. Jayakumari S, Ravichandiran V, Velraj M, Singh AK, Lakshmi AV. Anti-inflammatory activity of AP Linn. leaves Journal of Natural Remedies. 2012;12(1):56-62.
  11. 11. Moniruzzaman Ambia K, Mohammad ZI. Evaluation of antinociceptive activity of ethanol extract of leaves of AP. Evid Based Complement Alternat Med. 2015:1-8.
  12. 12. Muhammad Mohtasheem UH, Iqbal AS, Muzammil Salman A, Syed WA. Anti- emetic activity of some leguminous plants. Pak J Bot. 2012;12(1):389-39.
  13. 13. Mohana D, Thippeswamy S, Abhishek R, Manjunath K. Evaluation of antibacterial and antioxidant properties of some traditional medicinal plants from India. Int J Green Pharm. 2015;9(1):50-7. doi: 10.4103/0973-8258.150925.
  14. 14. Renilda Sophy AJ, Fleming AT. Evaluation of Adenanthera pavonina Bark extracts for antioxidant activity and cytotoxicity against cancer cell lines. Int J Sci Eng Res. 2015;4(12):48-51.
  15. 15. Muthu Kumaran P, Saraswathy N, Naga Mallikarjuna Raja B, Pravalika A. Anti- arthritic and antioxidant activity of aqueous and ethanolic extracts of Adenanthera pavonina Leaves. Inventi - planta Activa. 2013.
  16. 16. Wallis TE. Analytical Microscopy – Its aims and methods in relation to foods, water, spices and drugs. 3rd ed. Boston: little, brown & company; 1965.
  17. 17. Indian Pharmcacopeia. Govt of India. Vol. II, Appendices 91-98.
  18. 18. Harborne JB. Phytochemical methods, A guide to modern techniques of plant analysis. 3rd ed. New Delhi: Springer (India) private limited; 1998. p. 43-6.
  19. 19. Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteu reagent. Methods Enzymol. 1999;299:152-78. doi: 10.1016/S0076-6879(99)99017-1.
  20. 20. RabiaNaz AB. Phytochemical screening, antioxidant and antimicrobial potential of Lanata camarain different solvents. Asian Pac J Trop Dis. 2013;3(6):480-6.
  21. 21. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999;64(4):555-9. doi: 10.1016/S0308-8146(98)00102-2.
  22. 22. Rana MG. In-vitro anti-oxidant and free radicals scavenging studies of alcoholic extract of Medicago sativa L. 1996;55(1):15-22.
  23. 23. Marcocci L, Maguire JJ, Droyl-lefaix MT, Packer L. The nitric oxide scavenging properties of Ginkgo biloba extract. Biochem. Biophy. Res. Commum. 1994;201:748-55.
  24. 24. Prieto P, Pineda M, Anguilar M. Spectrophometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biocom. 1999;269:337-41.