Preparation and Evaluation of Aceclofenac and Piperine Loaded Solid Lipid Nanoparticles Using Box- Behnken Design

Abstract

The Aim of present study was preparation and evaluation of loaded Solid Lipid Nanoparticles using Box- Behnken Design. The three-component, three-level Box Behnken design, which was ideal for examining quadratic response surfaces and creating polynomial models with Design-Expert software (Version 13), was used to explore the influence of factors on solid lipid nanoparticles performance and features. The SLNs were prepared by Ultra sonication method using Vanaspati ghee and VCO as lipid, Glyceryl monostearate used as an emulsifier, Polysorbate 80 as surfactant and emulsifier, Carbopol 940 as polymer. The SLNs were evaluated for its Particle size, Polydispersity Index, Zeta potential, Entrapment Efficiency and In vitro % drug release. The FTIR studies revealed no chemical interaction between the drug molecule and polymers and found that drug was compatible with used polymer. Aceclofenac and Piperine loaded SLN formulation predicted by software consisted of 250 mg lipid, 600µl surfactant and 250 mg extract which on characterization studies found to have mean particle size of 85.5±8.2 nm, PDI of 0.342±0.06, zeta potential -13.5 mV, % EE (Ace) 82.73±2.89%, and %EE (Pip) 84.01±3.92%, which were in close agreement with the predicted values. The developed optimized formulation exhibited sustained drug release (Ace and Pip) for 24 h duration to the extent of 85.27±5.6% and 56.04±3.68% it was concluded that the Aceclofenac And Piperine loaded SLN based gel formulation containing carbopol was suitable for topical application and shows much better result in Rheumatoid arthritis (RA),