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Abstract

Hibiscus vitifolius also known as tropical rose mallow and grape leaved mallow, belongs to the family of Malveceae. It is a found in India and in United States of America. Hibiscus vitifolius is traditionally used for the treatment of jaundice, diabetics, inflammation, urease activity in India. The literature look over revealed the presence of alkaloids, flavones, gossypin, carotenoids, atropine, promethazine, aldose, galactose, bioflavonoids, morphine, pentahydroxy glycosyl flavones. The plant exhibited antibacterial, antimicrobial, antioxidant, antihepatotoxic, anticancer, diuretic, anti-inflammatory and antiepilepsy effect. The fresh leaves of Hibiscus vitifolius Linn were authenticated, collected, shade dried and coarsely powdered, was extracted with hydroalcohol. The extract was concentrated and stored in air tight container for further use. The aim of the present research study was to carry out for the identification of bioactive moleculess from hydroalcoholic extract of Hibiscus vitifolius Linn leaves by Gas chromatography- Mass spectrometry (GC-MS). GC-MS chromatogram showed twentyfive bioactive molecules which may attribute to different pharmacological properties.

Keywords

Gas chromatography- Mass spectrometry Hibiscus vitifolius (L)

Article Details

How to Cite
A. Krishnaveni, K. Umamageshwari, S. Prithivirajan, M. Bairavi, & T. Venkata Rathina Kumar. (2024). Gas Chromatography-Mass Spectrometry analysis of Hydroalcoholic extract of Hibiscus vitifolius Linn Leaves. International Journal of Research in Pharmacology & Pharmacotherapeutics, 13(3), 350-356. Retrieved from https://ijrpp.com/ijrpp/article/view/566

References

  1. 1. https://efloraofindia.com/2011/03/06/hibiscus-vitifolius/ Accessed on 02/092024
  2. 2. Kundu B.C and Chhabi Biswas 1974: On fioria vitifolia(L) mattei:Botanical survey of India, Calcutta: vol, 16,(1-4) 125-138.
  3. 3. Vijayan, P., Raghu, C., Ashok, G., Dhanaraj, S.A., Suresh, B., 2004. Antiviral activity of medicinal plants of Nilgiris. Indian Journal of Medical Research 120, 24–29
  4. 4. Jayalekshmi C.V, Reshma K. Ramesh, M. Vijai, and V. Suresh 2023: Ethnomedicinal Plants Used by Irula Tribal Settlement of Attappady in Palakkad District, Kerala, India: Bioprospecting of Tropical Medicinal Plant: pp 107–122(2023).
  5. 5. Johnson.M, Maharajan.M, Janakiraman. N 2015: Floristic diversity and medicinal importance of South Vagaikulam Region Tirunelveli, Tamil Nadu, South India: Journal of Medicinal Herbs and Ethnomedicine: 1 125-129.
  6. 6. S.Shanmugam, M.Kalaiselvan, P.Selvakumar, Kuru suresh and K.Rajendran 2011:Ethnomedical plants used to cure diarrhoea and dysentery sivagangai district of Tamilnadu,India : International journal of research in ayurveda &pharmacy :2(3) 991-994 .
  7. 7. Usha Devi, Himanshu Dwivedi, Aminudin and Hakimudin Khan. Traditional Phytotherapy of Bhadrak, Odisha – A Contribution: Hippocratic journal of unanimedicine: 2015; 11(3): 131-149.
  8. 8. Alshaimaa Hassan-Abdallah , Ali Merito , Souad Hassan , Djaltou Aboubaker , Mahdi Djama, Zemede Asfaw , Ensermu Kelbessa. Medicinal plants and their uses by the people in the Region of Randa, Djibouti: Journal of Ethnopharmacology. 2013: 148: 701–713.
  9. 9. Mahalekshmi K, Senthil Kumar N, Kishor KumarV: Pharmacognostical investigation of fioria vitifolia: International Journal of Biological & Pharmaceutical Research:2013; 4(4): 231-236.
  10. 10. Chamundeeswari .D,, Ethirajan Sukumar , Amar K , Brahmananda Reddy.N , Anil Kumar.I , Chowdry B.V, SamathaK. Cytotoxic and antibacterial activity of gosyypin :Natural products science: 2007; 13(4): 300-303.
  11. 11. Mahalakshmi.K, Senthilkumar.N, Solairaj.P, Parthiban K. Isolation of triterpenes from fioria vitifolia (l.) And it’s antioxidant activity: International Journal of Biological & Pharmaceutical Research: 2013; 4(9): 3596-3600,
  12. 12. Krishnamoorthi Mahalakshmi, Senthil Kumar Natesan, Kugalur Ganesan Parthiban, Ganesan Sekar. Anti-inflammatory and antipyretic activity of Fioria vitifolia : International Current Pharmaceutical Journal: 2013; 2(7): 119-121,
  13. 13. Pradeep Kumar Sama: Assessment of Hepatoprotective Activity of Hibiscus vitifolius leaves in CCL4 induced hepatotoxic rats: Research J. Pharmacology and Pharmacodynamics: 2013; 5(6): 313-316.
  14. 14. Pradeep Kumar Samal: Investigation of Antiepileptic Activity of Hibiscus vitifolius leaves: Asian J. Pharm. Tech: 2013; 3(2) 63-66.
  15. 15. Prabhakara.D, Rajeshkanna A, Senthamilselvi M.M. In vitro Anticancer Activity of Hibiscus vitifolius Flowers Ethyl Acetate Fraction against Hepg2 Cell Line: British Journal of Pharmceutical Research: 2016; 9 (5): 1-5.
  16. 16. Gayatri Jejurkar, Machindra Chavan 2023: Antimicrobial activity confirmed in phytoconstituent, Gossypin extracted from Hibiscus vitifolius Linn: Eur. Chem. Bull:12(8) 115-137.
  17. 17. https://www.ottokemi.com/fluorinated-building-blocks/3-Chloro4-Fluoroaniline.aspx#:~:text=C%202101%20(OTTO)%203%2D,important%20intermediates%20for%20Norfloxacin%20Synthesis: Accessed on 12.08.2024.
  18. 18. Herman G. Richey, Jr., (l) and Alan M. Rothman 1968: Intramolecular cyclizations of acetylenic grignard reagents: Tetrahedron Letters: 1457-1460. https://doi.org/10.1016/S0040-4039(01)98978-6
  19. 19. Klein SJ 2014: 1,3-Dinitrobenzene: Encyclopedia of Toxicology:volume 2. http://dx.doi.org/10.1016/B978-0-12-386454-3.01220-3
  20. 20. Desarbre.E, Savelon.L, Cornec. O, and Mdrour J.Y 1996: Oxidation of Indoles and 1,2-Dihydro-3H-indol-3-ones: Pergamon: Vol. 52, No. 8, pp. 2983-2994. https://doi.org/10.1016/0040-4020(96)00001-4
  21. 21. Sabrin R. M. Ibrahim, Sana A. Fadil, Haifa A. Fadil, Bayan A. Eshmawi, Shaimaa G. A. Mohamed and Gamal A. Mohamed 2022: Fungal Naphthalenones; Promising Metabolites for Drug Discovery: Structures, Biosynthesis, Sources, and Pharmacological Potential:Toxins : 14, 15. https://doi.org/10.3390/toxins14020154
  22. 22. https://en.m.wikipedia.org/wiki/Butyramide#:~:text=reduction%20of%20butyraldoxime.-,Derivatives,differentiation%20status20%20of%20most%20cells : Accessed on :13.08.2024.
  23. 23. Mehtap tugrak, halise inci gül, barış anil, ilhami gülçin 2020: Synthesis and pharmacological effects of novel benzenesulfonamides carrying benzamide moiety as carbonic anhydrase and acetylcholinesterase inhibitors: Turkish Journal of Chemistry: 44: 1601-1609. doi:10.3906/kim-2007-37
  24. 24. https://en.m.wikipedia.org/wiki/Myrtenal, Accessed on :13.08.2024
  25. 25. Dabin Choi, Wesuk Kang and Taesun Park 2020: Anti-Allergic and Anti-Inflammatory Effects of Undecane on Mast Cells and Keratinocytes:molecules : 2020; 25, 1554. doi:10.3390/molecules25071554
  26. 26. Goraksh J Hase, Keshav K Deshmukh, Raghunath D Pokharkar, Tukaram R Gaje, Narendra D Phatanagre 2017: Phytochemical Studies on Nerium oleander L. Using GC-MS: International Journal of Pharmacognosy and Phytochemical Research: 2007; 9(6); 885-891. Doi:10.25258/phyto.v9i6.8195
  27. 27. Sirigiri Chandra Kala and Kandru Ammani 2017: GC–MS analysis of biologically active compounds in Canthium parviflorum Lam. leaf and callus extracts: International Journal of ChemTech Research: 2017; 10(6): 1039-1058.
  28. 28. https://www.benchchem.com/product/b7830185 Accessed on 13.08.2024.
  29. 29. Archana Kumaria, Rajesh K. Singh: Morpholine as ubiquitous pharmacophore in medicinal chemistry: Deep insight into the structure-activity relationship (SAR): Bioorganic Chemistry: 2020; 96:10357. https://doi.org/10.1016/j.bioorg.2020.103578
  30. 30. Ishmam I. Arabi, Md Ahad Hossain, Sarkar M.A. Kawsar, Neha Joshi, Jyoti Maithani Kalra, Nidhi Gairola, Jyotsana Suyal, Amit Semwal, Rahadian Zainul, Vikash Jakhmola, Supriyo Saha 2024: Glucopyranoside Derivatives as Antibacterial and Antifungal Agents: QSAR, Molecular Docking and ADMET Analyses: Advanced Journal of Chemistry, Section A. 2024; 7(6): 758-776.
  31. Doi: 10.48309/AJCA.2024.462869.1555
  32. 31. Akimoto, Keng: Europaisches Patentamt European Patent Office Office europeen des brevets. European Patent Specification: 2004. EP 0 681 788.
  33. 32. Subramani, Meenashi, and Umamaheswari Sepperumal: GCMS Analysis of Pseudomonas sp., mediated degradation of polystyrene.:Ann Biol Re. 2017; 8.3 : 8-11.
  34. 33. Khalid A. Khan, Hassan M. Faidallah Abdullah M. Asiri 2013: Synthesis and Biological Evaluation of New N, N -Bis(1-substituted-ethylidene)-ethane1,2-diamine and Their Acetyl and Trifluoroacetyl Derivatives as Cytotoxic and Antimicrobial Agents: Hindawi Publishing Corporation Journal of Chemistry: 2013, Article ID 478635, 9 10. https://doi.org/10.1155/2013/478635
  35. 34. Josiah J. Bonire, Simon P. Fricker 2001: The in vitro antitumour profile of some 1,2-diaminocyclohexane organotin complexes:journal of inorganic biochemistry. 2001; 83: 217–221. Doi: 10.1016/s0162-0134(00)00192-6
  36. 35. Nashwa hafez zaher, mohammed ismail mostafa abdullah yousef altaher 2020: Design, synthesis and molecular docking of novel triazole derivatives as potential CoV helicase inhibitors: Acta Pharm: 1;70(2):145-159. DOI: 10.2478/acph-2020-0024
  37. 36. Shahab Khan 2024: Exploring the Chemistry, Reactions, Applications, and Biomedical Potentials of 2-Nitrobenzaldehyde and 2-Chlorobenzaldehyde: Fine Chemical Engineering: Volume 5 Issue 2 345 DOI:10.37256/fce.5220244858.
  38. 37. Olufunmiso Olusola Olajuyigbe, Tolulope Esther Onibudo, Roger Murugas Coopoosamy, Anofi Omotayo Tom Ashafa and Anthony Jide Afolayan 2018: Bioactive Compounds and in vitro Antimicrobial Activities of Ethanol Stem Bark Extract of Trilepisium madagascariense DC: International Journal of Pharmacology: 14 (7): 901-912 DOI:10.3923/ijp.2018.901.912.