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Abstract

A novel vaccine delivery system was developed for tetanus toxoid and HBsAg by formulating PLGA microspheres and surface modified by Chitosan. The produced microspheres were stabilized with various proteins and based on the results obtained the ideal formulation was optimized. The optimized formulation of TT containing microspheres and HBsAg containing microspheres were further evaluated for its immunological response. The immune response of the TT microspheres PCMS-TT was studied by immunizing guinea pigs with alum adsorbed vaccine (single dose), alum adsorbed vaccine with booster dose, PCMS-TT with trehalose and Mg (OH) 2, PCMS-TT with trehalose PCMS-TT with Mg (OH) 2. The immune serum of the above antigen was collected and used to treat the toxin induced mice. The PLGA –Chitosan Microspheres containing TT stabilized with trehalose and Mg (OH) 2 has produced higher levels of Ig G than the alum adsorbed tetanus vaccine with booster dose.  The Immune response of the HBsAg microspheres PCMS-HB was studied by immunizing guinea pigs. The concentration of anti-HBsAg antibody in the collected blood was then determined using the solid phase enzyme linked immunoassay (ELISA). The PLGA –Chitosan Microspheres containing HBsAg stabilized with trehalose and Mg (OH) 2 has produced almost equivalent IgG levels of the alum adsorbed hepatitis B vaccine with booster dose.

Keywords

Hepatitis B Vaccine Chitosan Single shot vaccine PLGA microspheres HBsAg Enzyme Immuno Assay

Article Details

How to Cite
V.Balasubramaniam, & K.S. Jaganathan. (2021). Immune response of novel carrier based vaccine delivery system for Tetanus Toxoid and r-HBsAg as single shot Vaccine. International Journal of Research in Pharmacology & Pharmacotherapeutics, 10(2), 181-188. https://doi.org/10.61096/ijrpp.v10.iss2.2021.181-188

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