Main Article Content


Aim and Objectives

This study is aimed to screen the anti-cancer action of naringenin in 7, 12-dimethyl benzanthracene induced breast cancer in female Wistar albino rats.

Materials and Methods

The study was conducted in the Division of Pharmacology, Annamalai University, Tamil Nadu. Female Wistar Albino rats weighing 140-150gm were selected in this study. All the animals were kept in Central Animal House under standard conditions. Total 36 rats were divided into six groups, each of 6 rats. G-I (Control), G-II- (7,12-Dimethyl benzanthracene (25 mg/kg/BW/sc), G-III (7,12-Dimethyl benzanthracene (25 mg/kg/sc)+Vincristine (500 µg/kg/BW/ip), G-IV (7,12-Dimethyl benzanthracene (25 mg/kg/sc)+Naringenin (50 mg/kg/BW/PO), G-V (7,12-Dimethyl benzanthracene (25 mg/kg/sc)+Naringenin (100 mg/kg/BW/PO) and G-VI (7,12-Dimethyl benzanthracene (25 mg/kg/sc)+Naringenin (200 mg/kg/BW/PO). Inducing drug was administered only once. All the drugs were given upto 16 weeks and blood samples were collected to estimate the anti-oxidant enzymes like TBARS, Super oxide dismutase, Catalase, Glutathione and Glutathione peroxidase.


Group-II showed significant changes compared to group-I. Group-III and V showed significant difference compared to Group-II. Group-V did not show significant difference compared to Group-III in TBARS, super oxide disumutase, catalse, glutathione and glutathione peroxidase enzymes.


Naringenin (100 mg/kg/BW/) showed significant anti-cancer effect compared to other doses.


Anti-cancer Anti-oxidants Naringenin vincristine 7, 12-Dimethyl bensanthracene Plasma

Article Details

How to Cite
Sandeep V M, Nirmala P, & SarathBabu K. (2021). Screening of anti-cancer effect of naringenin in 7, 12-dimethyl benzanthracene induced breast cancer in female Wistar Albino rats. International Journal of Research in Pharmacology & Pharmacotherapeutics, 7(4), 375-380. Retrieved from


  1. [1]. RadhaMunagala, Farrukh Aqil, Ramesh C.Gupta. Promising molecular targeted therapies in breast cancer.Indian j Phamacol.43(3), 2011, 236-45.
  2. [2]. American cancer society. Breast cancer: cancer facts and figures. Atlanta. American cancer society; 1995.
  3. [3]. GanesanDhamodharan, SankaranMirunalini. Dose response study of AgaricusBisporus (white button mushroom) and its Encapsulated chitosan Nanoparticles against 7, 12 –Dimethylbenz (a) anthracene induced mammary carcinogenesis in Female Sprague-Dawley rats .International journal of Pharmacy and Pharmaceutical sciences4(4), 2012, 20-25.
  4. [4]. NatlaSashidhar Reddy, Parthasarathy Nirmala, Natesan Chidambaram, Pammi Ashok. Quercetin in Dimethyl Benzanthracene Induced Breast Cancer in Rats. American journal of Pharmacology and Toxicology7(2), 2012, 68-72.
  5. [5]. Ferlay J BF, Pisani P, Parkin DM. GLOBOCAN 2000: Cancer Incidence, Mortality and Prevalence Worldwide, Version 1, 2001.
  6. [6]. Wilcox LJ, Borradaile N, Kurowska E, Telford DE, Huff MW. Naringenin, a citrus flavonoid, markedly decreases apoB secretion in HepG2 cells and inhibits acyl CoA:cholesterol acyltransferase. Circulation98, 1998, 1–537.
  7. [7]. Ameer B, Weintraub RA, Johnson JV, Yost RA, Rouseff RL. Flavanone absorption after naringin, hesperidin, and citrus administration. ClinPharmacolTher 60, 1996, 34–40.
  8. [8]. Leonardi T, Vanamala J, Taddeo SS, .Apigenin and Naringenin suppress colon Carcinogenesis through the aberrant crypt stage in azoxymethane –treated rats .ExpBiol Med (Maywood)235(6), 2010, 710-7.
  9. [9]. Travis LB, Hill D, Dores GM, et al. Cumulative absolute breast cancer risk for young women treated for Hodgkin lymphoma. J Natl Cancer Inst 97, 2005, 1428-37.
  10. [10]. Qin L, Jin L, Lu L, Lu X, Zhang C, Zhang F, Liang W. Naringenin reduces lung metastasis in a breast cancer resection model. Protein Cell2(6), 2011, 507-16.
  11. [11]. Cerutti PA: Oxy radicals and cancer. Lancet 344, 1994, 862-863.
  12. [12]. Oberley LW and Buettner GR: Role of superoxide dismutase in cancer- A review. Cancer Res39, 1979, 1141-49.
  13. [13]. Cavia-Saiz M, Busto MD, Pilar-Izquierdo MC, Ortega N, Perez-Mateos M, Muniz P. Antioxidant properties, radical scavenging activity and biomolecule protection capacity of flavonoid naringenin and its glycoside naringin: A comparative study. Journal of the Science of Food and Agriculture90, 2010, 1238–44.