Evaluation of Cardioprotective activity of Poly herbal extract on Dox and ISO induced Cardiac toxicity in Albino rats

Abstract

The activity of the poly herbal extract was studied by inducing cardiotoxicity with cumulative dose administration of Doxorubicin (15mg/kg i.p.for 2 weeks). The poly herbal extract was administered in doses of 200, 400 mg/kg b.w. for 15 days. In another model Isoproterenol induced myocardial infarction (necrosis) in rats. Animals were treated with poly herbal extract in doses of 200, 400 mg/kg b.w. for 30 days. On 31^st, 32^nd days the animals were treated with Isoproterenol (85mg/kg b.w. s.c.) to induce myocardial infarction. The general observations were serum myocytes biomarkers like LDH, CPK, biochemical parameters such as AST, ALT, total protein, and histopathology were done. It was found that in the invitro antioxidant activity the poly herbal extract of 9^th combination which include a ratio as (125:20:60:80) has shown more percentage inhibition. The administration of Dox and ISO induced cardiotoxicity associated with increased level of biomarkers. Pretreatment with the polyherbal extract significantly protected myocardium from the toxic effects of Dox and ISO by reducing elevated levels of biomarker enzymes like LDH, CPK, and biochemical parameters such as AST, ALT, total protein were back to normal.

Keywords: Cardiotoxicity, Doxorubicin, Isoproterenol, Polyherbal extract.