Cardioprotective Potential of A Polyherbal Combination Of Tinospora Cordifolia, Boerhaviadiffusa, And Glycyrrhiza Glabra Against Doxorubicin-Induced Cardiotoxicity

Abstract

Cardiovascular diseases remain a leading cause of global morbidity and mortality, with drug-induced cardiotoxicity posing a significant clinical challenge. The present study investigates the cardioprotective potential of a polyherbal combination consisting of Tinospora cordifolia, Boerhavia diffusa, and Glycyrrhiza glabra against doxorubicin-induced cardiotoxicity. The plant materials were collected, authenticated, and subjected to extraction using suitable solvents, followed by phytochemical screening and pharmacological evaluation. In vitro antioxidant activity of the polyherbal extract was assessed using lipid peroxidation, nitric oxide scavenging, DPPH free radical scavenging, and hydroxyl radical scavenging assays. Among various combinations, the optimized ratio demonstrated significant antioxidant potential. Acute toxicity studies confirmed the safety of the extract up to 2000 mg/kg, and doses of 200 mg/kg and 400 mg/kg were selected for in vivo studies. Cardioprotective activity was evaluated using doxorubicin-induced myocardial toxicity models in Wistar rats. Treatment with the polyherbal extract resulted in a significant reduction in serum cardiac biomarkers such as creatine phosphokinase (CPK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total protein levels compared to disease control groups. Histopathological examination further confirmed the protective effects, showing preservation of myocardial architecture and reduced cellular damage. The results suggest that the polyherbal formulation possesses significant cardioprotective activity, likely mediated through its antioxidant properties and ability to mitigate oxidative stress. This study supports the therapeutic potential of plant-based formulations in managing drug-induced cardiotoxicity.