Formulation and Evaluation of Face Serum Using Guava Leaf Extract

Abstract

The present study was conducted to formulate and evaluate a herbal face serum containing Psidium guajava (guava) leaf extract, a natural source of antioxidants and antimicrobial agents. Guava leaves contain potent bioactive compounds such as flavonoids, tannins, and phenolic compounds that contribute to skin rejuvenation and protection. Ethanolic extract of guava leaves was prepared using the Soxhlet extraction method and incorporated into a biphasic oil-in-water serum. The serum was formulated using flaxseed gel, glycerine, grape seed oil, sandalwood oil, Tween 80, triethanolamine, sodium benzoate and rose water.

Three formulations (F1–F3) were evaluated for their physical appearance, pH, viscosity, spreadability, microbial contamination, and stability. The optimized formulation (F2) showed a pH of 5.69, viscosity of 8.402 Pa·s, smooth texture, homogeneity and excellent spreadability. Stability studies confirmed no significant physical or chemical changes. The formulated serum exhibited good cosmetic acceptability and non-irritant properties, indicating its potential as a safe, effective, and natural skincare product. .