Main Article Content
Abstract
Controlled drug release system is one of the most favorable technique of novel drug delivery system owing to its reproducibility and ease of formulation. Nanotechnology is very useful for controlling the drug release and thus improving the pharmacokinetic and pharmacodynamic properties of the drug. The technique improves patient compliance by reducing both dose and the frequency of administration and thus minimizing the local as well as systemic toxic effects. The aim of the present research work was to formulate and evaluate nanoparticles of Tulasi by using the Emulsion solvent evaporation method. Sustained release nanoparticles of Tulasi were prepared to increase the drug residence time in gastrointestinal tract and thus improving the bioavailability of drug. The nanoparticles were prepared by using Chitosan and Carbopol940 as polymers. Different formulations were prepared with varying concentrations of Chitosan and Carbopol940in order to achieve the optimum particle size and maximum encapsulation efficiency. The particle size of nanoparticles was found to be in the range of 0.181±0.051 nm to 0.390±0.101 nm. Drug encapsulation efficiency ranged between 58.1±0.651 percent to 82.9±1.216% with controlled drug release up to 99.29% in phosphate buffer pH 6.8, 12 hrs. FT-IR studies showed that the drug and polymers were compatible. The results ofNanoparticles indicated that optimized formulation exhibited excellent properties.Nanoparticles in the doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg were used in Wistar rats of either sex. Were measured by standard biochemical methods. Silymarin (500 mg/kg) was used as a standard drug for assessment of Anti-Microbial status.When compared with the standard Anti-Microbial agent Ciprofloxacin, The antibacterial activity of these prepared nanoparticles against pathogenic bacterium has shown. The observed results suggest that Tulasi loaded Nanoparticles could be a potential source of Anti-Microbial Activity. However, further studies are required to explore this therapeutic property of Tulasi.
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References
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References
1. Hasan S. A Review on Nanoparticles : Their Synthesis and Types Biosynthesis: Mechanism. 2015; 49-11
2. Assessment R. Nanoparticles in the Environment 2007.
3. Cho E J, Holback H, Liu K C, Abouelmagd S A, Park J and Yeo Y. Nanoparticle characterization : State of the art, challenges, and emerging technologies. 2013.
4. Machado S, Pacheco J G, Nouws H P A, Albergaria J T and Delerue-Matos C. Characterization of green zero-valent iron nanoparticles produced with tree leaf extracts Sci. Total Environ. 2015; 533 76–81.
5. Renu Tiruwa. A review on nanoparticles – preparation and evaluation parameters. Indian J. Pharm. Biol. Res. 2015; 4(2):27-31
6. Abhilash M., Potential applications of Nanoparticles, International Journal of Pharma and Bio Sciences 2010; 1:1: 1-12.
7. Nagavarma B.V.N., Hemant K. S. Yadav, Ayuz A., Vasudha L.S., Shivakumar H.G, Different techniques for preparation of polymeric nanoparticles –A Review, Asian Journal of Pharmaceutical and Clinical Research 2012; 5:3: 1-8.
8. A. R. Mullaicharam, Nanoparticles in drug delivery system, International Journal of Nutrition, Pharmacology Neurological Diseases 2011; 1:2: 103-121.
9. Langer R. Biomaterials in drug delivery and tissue engineering; one labortory’s experience. Acc ChemRes.2000;33:94-101.
10. Bhadia D, Bhadra S, Jain P and Jain NK. Pegnology; a review of PEGylated systems; Pharmazin. 2002;57:5-20.
11. Tiwari D K, Behari J and Sen P 2008 Application of Nanoparticles in Waste Water Treatment 3 417–33
12. Salavati-niasari M, Davar F and Mir N 2008 Synthesis and characterization of metallic copper nanoparticles via thermal decomposition Polyhedron 27 3514–8
13. Tai C Y, Tai C, Chang M and Liu H 2007 Synthesis of Magnesium Hydroxide and Oxide Nanoparticles Using a Spinning Disk Reactor 5536–41
14. Bhaviripudi S, Mile E, Iii S A S, Zare A T, Dresselhaus M S, Belcher A M and Kong J 2007 CVD Synthesis of Single-Walled Carbon Nanotubes from Gold Nanoparticle Catalysts 1516–7.
15. Joachim Allouche, Synthesis of Organic and Bioorganic Nanoparticles: An Overview of the Preparation Methods, Springer-Verlag London 2013; 27-30.