Role of the card 15 gene, R702w mutation in inflammatory bowel disease individuals

Abstract

Digestive problems are among the most common conditions affecting population today. There are many different
types of digestive problems, from gastrointestinal infections that make a person miserable surpass quickly to long
term illness like IBD. IBD is a general term that refers to illness that causes chronic inflammation in the intestines.
IBD consists mainly of 2 conditions. (1) Ulcerative colitis and (2) Crohn`s disease.
CARD15 wild type protein mediates responsiveness to lipopolysaccharide (LPS) and peptidoglycan (PGN)
preparations from several bacteria. Recent works identified muramly dipeptide (MDP), the moiety specifically
recognized by CARD15. The 3 major crohn disease associated variants are defective in their ability to activate NFKB in response to MDP.
The main objective of this work is to screen the R702W mutation in IBD individuals by using methods like DNA
isolation, Amplification Refractory Mutation System (ARMS) (allele specific method) and electrophoresis. In this
present study, the results revealed that there is no R702W variant polymorphism in all selected 20 IBD individuals.