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Submitted
Apr 28, 2023
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Apr 28, 2023
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Abstract

Aspirin is an antiplatelet agent therefore it is made to develop a new simple, sensitive and cost-effective UV spectrophotometric technique for the estimating aspirin using Folin Ciocalteu has been devised. The approach is based on formation of blue colored Chromagen as a result of Folin-Ciocalteu reagent reacts with the phenolic groups in aspirin. The spectrum was produced, and the maximum absorbance was found at 750 nm, with the beers law obeying across the concentration range 2-20 µg/ml with correlation coefficient found to be 0.9997. The method was validated for various parameters such as accuracy, precision robustness. The %RSD of precision was found to be 0.94%. The limit of detection and quantification value are 0.43 and 1.31 µg/ml respectively. The results of the proposed method were statistically validated as per ICH Q2 R1 guidelines and results were found to be satisfactory.


 

Keywords

Aspirin, Folin-Ciocalteu reagent, UV Spectrophotometric method, Validation.

Article Details

How to Cite
A. Raja Reddy, N.Divya, P.Pravalika, S. Hashika Keerthana, & R.Kiran. (2023). Spectrophotometric Determination of Aspirin by Using FC Reagent. International Journal of Research in Pharmacology & Pharmacotherapeutics, 12(2), 93-97. https://doi.org/10.61096/ijrpp.v12.iss2.2023.93-97
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