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Submitted
Oct 28, 2022
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Oct 28, 2022
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Abstract

A new simple, accurate, economic, rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Valacyclovir in bulk form and its pharmaceutical dosage form. Chromatographic separation was carried out on Zorbax C18 (4.6mm x 250mm, 5mm, Make: X terra) column using a mixture of Acetonitrile: Methanol: Water (50:30:20% v/v) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 254 nm. The retention time of the Valacyclovir was found to be 5.462 ±0.02min. The method was validated according to ICH guidelines for linearity, sensitivity, accuracy, precision, specificity and robustness. The response was found to be linear in the drug concentration range of 50-90 mcg/mL for Valacyclovir.The correlation coefficient was found to be 0.999. The LOD and LOQ for Valacyclovir were found to be 1.6µg/mL and 4.8µg/mL respectively. The proposed method was found to be good percentage recovery for Valacyclovir, which indicates that the proposed method is highly accurate. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and marketed pharmaceutical formulations.

Keywords

Valacyclovir, RP-HPLC, Method Development, Validation, ICH Guidelines.

Article Details

How to Cite
Sameena Tabassum, K. Vamshi Krishna, & Ramya Sri. S. (2022). Analytical Method Development and Validation of Valacylovir in Pharmaceuticals and Bulk Dosage Form by Using RP-HPLC. International Journal of Research in Pharmacology & Pharmacotherapeutics, 11(4), 174-179. https://doi.org/10.61096/ijrpp.v11.iss4.2022.174-179
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