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Abstract
Desmodium triflorum, which is commonly known as “three-flowered beggar weed” is a dicotyledon, family Fabaceae. The present study was carried out to evaluate the immunomodulatory activity of aqueous extract of D. triflorum on Swiss albino rats. Delayed type hypersensitivity response and Humoral antibody titer by using injecting 0.1 ml of Sheep red blood cells (SRBCs) suspension was used to assess the activity of drug. Levamisole was given in the dose of 50 mg/kg body weight through oral route. 200 and 400 mg/kg body weight of Desmodium triflorum herbal drug was given through oral route. The extent of protection against SRBCs suspension was evaluated with drug administration by estimating total leucocyte and differential leucocyte count. aqeuous extract of D. triflorum showed pronounced immunostimulant activity by increasing the levels of total WBC count. The extract was found to be an effective immunostimulant agent.
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References
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References
1. ZA Mahmood, Mohammad Sualeh, Saad Bin Zafar Mahmood and Mahwish A K. Herbal treatment for cardiaovascular disease the evidence based therapy,Pak.J. pharm. Sci., 2010, vol.23, no.1, pg.no; 119-124.
2. ShivaprasadHN, KharyaMD, RanaAC, Mohan S. Preliminary immunemodulatory activities of aqueous extract of Terminaliachebula. Pharmaceutical Biology 2006;44,32-34.
3. U.A. Shinde, A. S. Phadka, A.M.Nair, A.A. Mungantiwar., “Preliminary studies on the Immunomodulatory activity of Cedursdeodara wood oil ”Fitoterapia. Vol. 70 (1999) p.no. 333- 3339.
4. Kokate CK. In: Practical Pharmacognosy, Preliminary Phytochemical Screening, first ed., Vallabh Prakashan, New Delhi, 1986; 111.
5. OECD/OCDE, OECD Guidelines for the testing of chemicals, revised draft guidelines 423: Acute Oral toxicity- Acute toxic class method, revised document, CPCSEA, Ministry of Social Justice and Empowerment. New Delhi: Government of India; 2000.
6. P. K. Mediratta, K.K. Sharma, Surrendersingh.”Evaluaiton of Immunomodulatory potential of Occiumsanctiumseed oil end its possible mechanism of action”, Journal of Ethnopharmacology Vol. 80 (2002) p.no. 15-20.
7. A.B. Gokhare, A.S. Damne, M.N. Sanad., “Investigation into the immunomodulatory activity of Argyreiaspeciosa”, Journal of Ethno Pharmacology Vol. 84(2003) p.no. 109-114.
8. E.S. Sunila, G.Kuttan., “Immunomodulatory activity and anti- tumor activity of Piper longumLinn and pipperine”, Journal of Ethnopharmacology Vol. 90 (2004) p.no. 339-.346.
9. ShahjahaM, Vani G. Shyamaladevics., “Effect of solanumtrilobatumon the antioxidanttsatusdurign diethyl nitrosamine induced and Phenobarbital promoted hepatocarcinogenesis in rat”, Chem Biol Interact. Vol. 17(20005) p.no. 113-123.
10. Shivaprasad HN, Kharya MD, Rana AC, Mohan S. Preliminary immunomodulatory activities of aqueous extract of Terminaliachebula. Pharmaceutical Biology 2006;44,32-34.