Main Article Content
Abstract
To test the analgesic and anti-inflammatory activity of different fractions of dried Tecoma stans leaves using various pain and inflammation models. The analgesic activity of T. stans was tested in mice with acetic acid-induced writhing and rats with the tail flick test. Cotton pellet-granuloma formation in rats was used to test the anti-inflammatory activity. T. stans was examined in five distinct fractions (FRI, FRII, FRIII, FRIV, and FRV) at doses of 20 and 40 mg/kg, p.o. When compared to the other fractions, the fractions FRI (40 mg/kg, p.o.) and FRIII (40 mg/kg, p.o.) were shown to be more efficacious (P0.01) in reducing cotton pellet granuloma development and acetic acid induced writhing. In the tail flick method, FRI (20 mg/kg, p.o.) and FRIII (20 mg/kg, p.o.) were likewise found to be more effective in increasing latency time. FRI and FRIII, two of the five fractions of T. stans leaves examined, have significant analgesic and anti-inflammatory properties against several types of inflammation and pain.
Keywords
Tecoma stans
granuloma formation
carrageenan
tail flick
acetic acid
Article Details
How to Cite
Screening of tecoma stans leaves fractions for analgesic and anti-inflammatory activities. (2021). International Journal of Research in Pharmacology & Pharmacotherapeutics, 10(4), 317-323. https://doi.org/10.61096/ijrpp.v10.iss4.2021.317-323
How to Cite
Screening of tecoma stans leaves fractions for analgesic and anti-inflammatory activities. (2021). International Journal of Research in Pharmacology & Pharmacotherapeutics, 10(4), 317-323. https://doi.org/10.61096/ijrpp.v10.iss4.2021.317-323
References
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1. Bohlin L. Structure-activity studies of natural products with anti-inflammatory effects. In: Hostettmann K, editor. Phytochemistry of Plants Used in Traditional Medicine. Oxford: Clarendon Press; 1995. p. 137-61.
2. Yesilada E, Ustun O, Sezik E, Takaishi Y, Ono Y, Honda G. Inhibitory effects of Turkish folk remedies on inflammatory cytokines: interleukin-1, interleukin-1 and tumor necrosis factor. J Ethnopharmacol 1997;58:59-73.
3. Robert A, Hanchar AJ, Lancaster C, Nezamis JE. Prostacyclin inhibits enteropooling and diarrhea. In: Vane JR, Bergström S, editors. Prostacyclin. New York: Raven Press; 1979. p. 147-58.
4. Miller TA. Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Am J Physiol 1983;245:601-23.
5. Parrotta J A Healing plants of Pennisular india. CABI publishing 2001;701-02.
6. K.N.V Rao et al. establishment of two varieties in tecoma stans ofindian origin pharmacolognostically and pharmacologically. J.Phytology 2010;2:92-102.
7. Dr.R.Manivannan, Mr.S.Kameshwaran, Mr.M.Sarbudeen, Mr.G.Sureshkumar, Mr.N.Elavarasan, Mrs.S.Harshini. Potency of Tecoma stans flowers on atherothrombosis (In Vitro). International Journal of Allied Medical Sciences and Clinical Research Vol-8(2) 2020 150-154.
8. Kameshwaran.S, Dr.N.Sriram, Dr. Rajaneeekar Dasari, Asok Kumar DS, Abhenaya.K, Antihyperlipidemic activity of flower extract of Tecoma stans against dexamethasone-induced hyperlipidemia in rats. Int. J. of Allied Med. Sci. and Clin. Research Vol-8(3) 2020, 622-628.
9. S.Kameshwaran, R.Sundara Ganapathy, S.Arun, C.Dhanapal, Effects of Tecoma stans extract on hepatic and cardiovascular disease (CVD) markers in Streptozotocin induced diabetic albino rats. International Journal of Pharmacological screening methods, Vol 5 / Issue 2 / 2015 / 107-112.
10. S.Kameshwaran, R.Sundara Ganapathy, S.Arun, C.Dhanapal. Effect of ethanolic extract of Tecoma stans on learning and memory in normal and memory deficit animals. Asian J. Pharm. Res. Vol 5, Issue 3, 236-241, 2015.
11. Khandelwal KR, Kokate CK, Pawar AP, Gokhle SR. Practical Pharmacognosy techniques and experiments. Pune: Nirali Prakshan; 1996. p. 9.
12. Davies OL, Raventos J, Walpole AL. Method for evaluation of analgesic activity using rats. British J Pharmacol 1946;1:255-64.
13. Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening. Proc Soc Exp Biol 1959;18:412-5.
14. Winter CA, Risley EA, Nuss GW. Carrageenan induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol 1962;111:544-7.
15. WinderCV, Wax J, Scothi T, Scherrer RA, Short FW. Antiinflammatory, Antipyretic and Antiniciceptive properties of N-(2-3 xylyl) anthranilic acid. J Pharmacol Exp Thera 1962;138:405-13.
16. Swingle KF, Shideman FE. Phase of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents. J Pharmacol Exp Thera 1972;183:226-34.
17. Amico-Roxus M, Caruso A, Trombadore S, Scifo R, Scapagini U. Gangliosides antinoceptive effects in rodents. Arch Int Pharmacodyn Ther 1984;272:117. Back to cited text no. 17
18. Raj PP. Pain mechanism in pain medicine: A comprehensive review. In: Raj PP, editor. 1 st ed. Missouri: mosby-year book; 1996. p. 23. Back to cited text no. 18
19. Franzotti EM, Santos CV, Rodrigues HM, Mourao RH, Andrade MR, Antoniolli AR. Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malva- branca). J Ethnopharmacol 2000;72:273-7.
20. Shuanglin H, Osamu T, Hiroshi I. Intrathecal endomorphin- I produces antinociceptive modulated by alpha 2 adrenoceptors in rat tail flick, tail pressure and formalin test. Life Sci 2000;6:204.
21. Hernandez-Perez M, Rabanal Gallego R. Evaluation of the antiinflammatoryand analgesic activity of Sideritis canariensis var.pannosa in mice. J Ethnopharmacol 2002;81:43-7.
22. Mehrotra BN, Rastogi RD. Compendium of Indian Medicinal Plants. Central Drug Research Institute, Lucknow and National Institute of Science Communication and Information Resources, New Delhi; 2004. p. 188.
23. Nadkarni KM. Indian Materia Medica. 3 rd ed. Bombay: Popular Prakashan Private Limited; 2002. p. 552.
24. Swami KD, Bisht NP. Constituents of Tecoma stansand F. infectoria and their biological activity. J Indian Chem Soc 1996;73:631.
25. Das PC, Das A, Mandal S. Antimicrobial and anti-inflammatory activities of the seed kernel of Mangifera indica. Fitoterapia 1989;60:235-40.
26. Ahmadiani A, Hosseiny J, Semnanian S, Javan M, Saeedi F, Kamalinejad M, et al. Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruitextract. J Ethnopharmacol 2000;72:287-92.
27. Srivastava RC, Hussain MM, Hassan SK, Athar M. Green tea polyphenols and tannic acid act as potent inhibitors of phorbol esters-induced nitric oxide generation in rat hepatocytes independent of their antioxidant properties. Cancer Lett 2000;153:1-5.
References
1. Bohlin L. Structure-activity studies of natural products with anti-inflammatory effects. In: Hostettmann K, editor. Phytochemistry of Plants Used in Traditional Medicine. Oxford: Clarendon Press; 1995. p. 137-61.
2. Yesilada E, Ustun O, Sezik E, Takaishi Y, Ono Y, Honda G. Inhibitory effects of Turkish folk remedies on inflammatory cytokines: interleukin-1, interleukin-1 and tumor necrosis factor. J Ethnopharmacol 1997;58:59-73.
3. Robert A, Hanchar AJ, Lancaster C, Nezamis JE. Prostacyclin inhibits enteropooling and diarrhea. In: Vane JR, Bergström S, editors. Prostacyclin. New York: Raven Press; 1979. p. 147-58.
4. Miller TA. Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Am J Physiol 1983;245:601-23.
5. Parrotta J A Healing plants of Pennisular india. CABI publishing 2001;701-02.
6. K.N.V Rao et al. establishment of two varieties in tecoma stans ofindian origin pharmacolognostically and pharmacologically. J.Phytology 2010;2:92-102.
7. Dr.R.Manivannan, Mr.S.Kameshwaran, Mr.M.Sarbudeen, Mr.G.Sureshkumar, Mr.N.Elavarasan, Mrs.S.Harshini. Potency of Tecoma stans flowers on atherothrombosis (In Vitro). International Journal of Allied Medical Sciences and Clinical Research Vol-8(2) 2020 150-154.
8. Kameshwaran.S, Dr.N.Sriram, Dr. Rajaneeekar Dasari, Asok Kumar DS, Abhenaya.K, Antihyperlipidemic activity of flower extract of Tecoma stans against dexamethasone-induced hyperlipidemia in rats. Int. J. of Allied Med. Sci. and Clin. Research Vol-8(3) 2020, 622-628.
9. S.Kameshwaran, R.Sundara Ganapathy, S.Arun, C.Dhanapal, Effects of Tecoma stans extract on hepatic and cardiovascular disease (CVD) markers in Streptozotocin induced diabetic albino rats. International Journal of Pharmacological screening methods, Vol 5 / Issue 2 / 2015 / 107-112.
10. S.Kameshwaran, R.Sundara Ganapathy, S.Arun, C.Dhanapal. Effect of ethanolic extract of Tecoma stans on learning and memory in normal and memory deficit animals. Asian J. Pharm. Res. Vol 5, Issue 3, 236-241, 2015.
11. Khandelwal KR, Kokate CK, Pawar AP, Gokhle SR. Practical Pharmacognosy techniques and experiments. Pune: Nirali Prakshan; 1996. p. 9.
12. Davies OL, Raventos J, Walpole AL. Method for evaluation of analgesic activity using rats. British J Pharmacol 1946;1:255-64.
13. Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening. Proc Soc Exp Biol 1959;18:412-5.
14. Winter CA, Risley EA, Nuss GW. Carrageenan induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol 1962;111:544-7.
15. WinderCV, Wax J, Scothi T, Scherrer RA, Short FW. Antiinflammatory, Antipyretic and Antiniciceptive properties of N-(2-3 xylyl) anthranilic acid. J Pharmacol Exp Thera 1962;138:405-13.
16. Swingle KF, Shideman FE. Phase of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents. J Pharmacol Exp Thera 1972;183:226-34.
17. Amico-Roxus M, Caruso A, Trombadore S, Scifo R, Scapagini U. Gangliosides antinoceptive effects in rodents. Arch Int Pharmacodyn Ther 1984;272:117. Back to cited text no. 17
18. Raj PP. Pain mechanism in pain medicine: A comprehensive review. In: Raj PP, editor. 1 st ed. Missouri: mosby-year book; 1996. p. 23. Back to cited text no. 18
19. Franzotti EM, Santos CV, Rodrigues HM, Mourao RH, Andrade MR, Antoniolli AR. Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malva- branca). J Ethnopharmacol 2000;72:273-7.
20. Shuanglin H, Osamu T, Hiroshi I. Intrathecal endomorphin- I produces antinociceptive modulated by alpha 2 adrenoceptors in rat tail flick, tail pressure and formalin test. Life Sci 2000;6:204.
21. Hernandez-Perez M, Rabanal Gallego R. Evaluation of the antiinflammatoryand analgesic activity of Sideritis canariensis var.pannosa in mice. J Ethnopharmacol 2002;81:43-7.
22. Mehrotra BN, Rastogi RD. Compendium of Indian Medicinal Plants. Central Drug Research Institute, Lucknow and National Institute of Science Communication and Information Resources, New Delhi; 2004. p. 188.
23. Nadkarni KM. Indian Materia Medica. 3 rd ed. Bombay: Popular Prakashan Private Limited; 2002. p. 552.
24. Swami KD, Bisht NP. Constituents of Tecoma stansand F. infectoria and their biological activity. J Indian Chem Soc 1996;73:631.
25. Das PC, Das A, Mandal S. Antimicrobial and anti-inflammatory activities of the seed kernel of Mangifera indica. Fitoterapia 1989;60:235-40.
26. Ahmadiani A, Hosseiny J, Semnanian S, Javan M, Saeedi F, Kamalinejad M, et al. Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruitextract. J Ethnopharmacol 2000;72:287-92.
27. Srivastava RC, Hussain MM, Hassan SK, Athar M. Green tea polyphenols and tannic acid act as potent inhibitors of phorbol esters-induced nitric oxide generation in rat hepatocytes independent of their antioxidant properties. Cancer Lett 2000;153:1-5.
2. Yesilada E, Ustun O, Sezik E, Takaishi Y, Ono Y, Honda G. Inhibitory effects of Turkish folk remedies on inflammatory cytokines: interleukin-1, interleukin-1 and tumor necrosis factor. J Ethnopharmacol 1997;58:59-73.
3. Robert A, Hanchar AJ, Lancaster C, Nezamis JE. Prostacyclin inhibits enteropooling and diarrhea. In: Vane JR, Bergström S, editors. Prostacyclin. New York: Raven Press; 1979. p. 147-58.
4. Miller TA. Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Am J Physiol 1983;245:601-23.
5. Parrotta J A Healing plants of Pennisular india. CABI publishing 2001;701-02.
6. K.N.V Rao et al. establishment of two varieties in tecoma stans ofindian origin pharmacolognostically and pharmacologically. J.Phytology 2010;2:92-102.
7. Dr.R.Manivannan, Mr.S.Kameshwaran, Mr.M.Sarbudeen, Mr.G.Sureshkumar, Mr.N.Elavarasan, Mrs.S.Harshini. Potency of Tecoma stans flowers on atherothrombosis (In Vitro). International Journal of Allied Medical Sciences and Clinical Research Vol-8(2) 2020 150-154.
8. Kameshwaran.S, Dr.N.Sriram, Dr. Rajaneeekar Dasari, Asok Kumar DS, Abhenaya.K, Antihyperlipidemic activity of flower extract of Tecoma stans against dexamethasone-induced hyperlipidemia in rats. Int. J. of Allied Med. Sci. and Clin. Research Vol-8(3) 2020, 622-628.
9. S.Kameshwaran, R.Sundara Ganapathy, S.Arun, C.Dhanapal, Effects of Tecoma stans extract on hepatic and cardiovascular disease (CVD) markers in Streptozotocin induced diabetic albino rats. International Journal of Pharmacological screening methods, Vol 5 / Issue 2 / 2015 / 107-112.
10. S.Kameshwaran, R.Sundara Ganapathy, S.Arun, C.Dhanapal. Effect of ethanolic extract of Tecoma stans on learning and memory in normal and memory deficit animals. Asian J. Pharm. Res. Vol 5, Issue 3, 236-241, 2015.
11. Khandelwal KR, Kokate CK, Pawar AP, Gokhle SR. Practical Pharmacognosy techniques and experiments. Pune: Nirali Prakshan; 1996. p. 9.
12. Davies OL, Raventos J, Walpole AL. Method for evaluation of analgesic activity using rats. British J Pharmacol 1946;1:255-64.
13. Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening. Proc Soc Exp Biol 1959;18:412-5.
14. Winter CA, Risley EA, Nuss GW. Carrageenan induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol 1962;111:544-7.
15. WinderCV, Wax J, Scothi T, Scherrer RA, Short FW. Antiinflammatory, Antipyretic and Antiniciceptive properties of N-(2-3 xylyl) anthranilic acid. J Pharmacol Exp Thera 1962;138:405-13.
16. Swingle KF, Shideman FE. Phase of the inflammatory response to subcutaneous implantation of a cotton pellet and their modification by certain anti-inflammatory agents. J Pharmacol Exp Thera 1972;183:226-34.
17. Amico-Roxus M, Caruso A, Trombadore S, Scifo R, Scapagini U. Gangliosides antinoceptive effects in rodents. Arch Int Pharmacodyn Ther 1984;272:117. Back to cited text no. 17
18. Raj PP. Pain mechanism in pain medicine: A comprehensive review. In: Raj PP, editor. 1 st ed. Missouri: mosby-year book; 1996. p. 23. Back to cited text no. 18
19. Franzotti EM, Santos CV, Rodrigues HM, Mourao RH, Andrade MR, Antoniolli AR. Anti-inflammatory, analgesic activity and acute toxicity of Sida cordifolia L. (Malva- branca). J Ethnopharmacol 2000;72:273-7.
20. Shuanglin H, Osamu T, Hiroshi I. Intrathecal endomorphin- I produces antinociceptive modulated by alpha 2 adrenoceptors in rat tail flick, tail pressure and formalin test. Life Sci 2000;6:204.
21. Hernandez-Perez M, Rabanal Gallego R. Evaluation of the antiinflammatoryand analgesic activity of Sideritis canariensis var.pannosa in mice. J Ethnopharmacol 2002;81:43-7.
22. Mehrotra BN, Rastogi RD. Compendium of Indian Medicinal Plants. Central Drug Research Institute, Lucknow and National Institute of Science Communication and Information Resources, New Delhi; 2004. p. 188.
23. Nadkarni KM. Indian Materia Medica. 3 rd ed. Bombay: Popular Prakashan Private Limited; 2002. p. 552.
24. Swami KD, Bisht NP. Constituents of Tecoma stansand F. infectoria and their biological activity. J Indian Chem Soc 1996;73:631.
25. Das PC, Das A, Mandal S. Antimicrobial and anti-inflammatory activities of the seed kernel of Mangifera indica. Fitoterapia 1989;60:235-40.
26. Ahmadiani A, Hosseiny J, Semnanian S, Javan M, Saeedi F, Kamalinejad M, et al. Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruitextract. J Ethnopharmacol 2000;72:287-92.
27. Srivastava RC, Hussain MM, Hassan SK, Athar M. Green tea polyphenols and tannic acid act as potent inhibitors of phorbol esters-induced nitric oxide generation in rat hepatocytes independent of their antioxidant properties. Cancer Lett 2000;153:1-5.