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May 17, 2021
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May 17, 2021
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Abstract

Aim:

To study the role of allopurinol as an oxygen free radical scavenger, in prevention of myocardial ischemic reperfusion injury in albino rabbits.


Materials and Methods

Albino Rabbits of either sex were divided into two groups control (n=5) and test group (n=5), receiving antioxidant allopurinol. Experiments were done using isolated heart perfusion apparatus (Langendorff apparatus). The experiment was divided into three phases perfusion (15 min) followed by ischemia (5 min) followed by reperfusion (15 min). LDH level of perfusate and coronary flow were taken as biochemical and physiological marker of myocardial reperfusion injury respectively were measured at 5, 10 and 15 min of post ischemic period. Control and test groups were compared.


Result:

The result shows that in the group receiving allopurinol the decrease in post ischemic LDH level was significant (p<0.01) at 5,10 and 15 min while there was significant increase in post ischemic coronary flow at 5 min (p<0 .05) as compared with control.


Conclusion

Antioxidant allopurinol has protective role in prevention of myocardial reperfusion injury in albino rabbits.


Aim:

To study the role of allopurinol as an oxygen free radical scavenger, in prevention of myocardial ischemic reperfusion injury in albino rabbits.


Materials and Methods

Albino Rabbits of either sex were divided into two groups control (n=5) and test group (n=5), receiving antioxidant allopurinol. Experiments were done using isolated heart perfusion apparatus (Langendorff apparatus). The experiment was divided into three phases perfusion (15 min) followed by ischemia (5 min) followed by reperfusion (15 min). LDH level of perfusate and coronary flow were taken as biochemical and physiological marker of myocardial reperfusion injury respectively were measured at 5, 10 and 15 min of post ischemic period. Control and test groups were compared.


Result:

The result shows that in the group receiving allopurinol the decrease in post ischemic LDH level was significant (p<0.01) at 5,10 and 15 min while there was significant increase in post ischemic coronary flow at 5 min (p<0 .05) as compared with control.


Conclusion

Antioxidant allopurinol has protective role in prevention of myocardial reperfusion injury in albino rabbits.

Keywords

Allopurinol Langendorff apparatus LDH (lactate dehydrogenase) Reperfusion injury

Article Details

How to Cite
G Gambhir, AK Gupta, IP Jain, P Agrawal, V Kushwaha, A Singh, AN Singh, P Tripathi, & A Tilak. (2021). Role of allopurinol as an oxygen free radical scavanger in prevention of ischemic reperfusion myocardial injury in albino rabbits. International Journal of Research in Pharmacology & Pharmacotherapeutics, 5(1), 70-75. https://doi.org/10.61096/ijrpp.v5.iss1.2016.70-75
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