Article Sidebar

Submitted
May 4, 2021
Published
May 4, 2021
Download
PDF
Statistic
Read Counter : 165 Download : 99

Main Article Content

Abstract

Sida acuta is shrub indigenous to pantropical areas, widely distributed in regions and found in pastures, waste lands, cultivated lands, roadsides, lawns and planted forests. Sida acuta is ethanomedically used as treatment of diuretic, asthma, fever, headache, cough, cold, ulcer, anthelmintic, snake bite, urinary disease, female disorders, sedative, eczema, kidney stone, elephantiasis, testicular swelling, poultice for dandruff, rheumatic affections, facial paralysis, pulmonary tuberculosis, gonorrheae. The phytochemical screening revealed the presence of tannin, saponin, flavonoid, terpenoids, cardio glycoside, vitamin composition was thiamine, niacin, ascorbic acid, tocopherol, riboflavin and mineral composition was calcium, magnesium, zinc, steroids, phenolic compounds, sesquiterpene, alkaloid cryptolepine, quindoline, quindolinone and fixed oil. The hydro alcohol extract was subjected to preliminary phytochemical analysis and the same was evaluated for its antioxidant studies by in vitro methods such as hydrogen peroxide scavenging activity, reducing power assay and total antioxidant capacity. The phytochemical analysis indicated the presence of alkaloids, carbohydrates, cardio glycoside, sterols, saponins, flavonoids,protein and aminoacids, terpenoids, resins, gum  mucilage, quinone and coumarin. The antioxidant studies concentration (IC50) of Sida acuta of hydrogen peroxide scavenging effect was found to be 1.83μg/mL in comparison with ascorbic acid (0.95μg/mL). Inhibitory concentration (IC50) of Sida acuta for reducing power effect is found to be 142μ/mL in comparison with ascorbic acid 15μg/mL and Inhibitory concentration (IC50) of Sida acuta for total antioxidant capacity was found to be 185μg/mL in comparison with ascorbic acid 159μg/mL. The present research draws the conclusion that this Sida acuta plant showed mild antioxidant effect, which may be due to the phytoconstituents.

Keywords

Antioxidant Malvaceae Phytochemical Sida acuta

Article Details

How to Cite
Krishnaveni A, Ezhilarasan B, Iyappan A, & Abdul Hasan Sathali A. (2021). Preliminary phytochemical screening and in vitro antioxidant activity of Sida Acuta Burm. International Journal of Research in Pharmacology & Pharmacotherapeutics, 7(2), 157-165. https://doi.org/10.61096/ijrpp.v7.iss2.2018.157-165
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. [1]. Mudaliar, M. Siddha Materia Medica (Medicinal plants Division) Department of Homeopathy, Directorate of Indian Medicines, Chennai. 1998, P 387.
  2. [2]. Ananil, K., Hudson, J.B., de Souzal, C., Akpaganal, K., Tower, G.H.N., Amason, J.T., Gbeassor, M. Investigation of Medicinal Plants of Togo for Antiviral and Antimicrobial activities. Togo, 38(1) 2000, 40-45.
  3. [3]. Silja, VP., Varma, SK., Mohanan, KV. Ethnomedicinal plant knowledge of the Mullukuruma tripe of Wayanad district, Kerala. Indian J.Trad.Knowl. 7(4), 2008, 604-612.
  4. [4]. Oliver Tene Tcheghebe, Armel Jackson Seukep , Francis Ngouafong Tatong. Ethanomedical uses, phytochemical and pharmacological profiles, and toxicity of Sida acuta Burm.f: A review article. The Pharma Innovation Journal.6(6), 2017, 01-06
  5. [5]. Saraswathy, A., Gnana, RR., Govindarajan, S., Kundu, AB. Chemical investigation of Sida acuta Burm. Bull. Med.Eth. Bot. Res, 19, 1998, 176-180.
  6. [6]. Nwankpa, P., Chukwuemeka, OG., Uloneme, GC., Etteh, CC., Ugwuezumba P. Phyto-nutrient composition and oxidative potential of ethanolic leaf extract of Sida acuta in wistar albino rats. A J Biotechnol. 14(49) 2015, 3264-3269.
  7. [7]. Konate, K., Souza A, Coulibaly, AY., Meda NTR., K irendrebeogo M. In vitro antioxidant, Lipoxygenase and xanthine oxidase inhibitory activities of fractions from Cienfuegosia DigitataCav. Sida alba L. and Sida acuta Burm f. (Malvaceae). Pak J Biol Sci. 13, 2010, 1092-1098.
  8. [8]. Jang, DS., Park, EJ., Kang, YH., Su BN, Hawthrone, ME., Vigo, JS., Graham, JG., Cableses, F., Fong, HH., Mehta, RG., Pezzuto, JM., Kinghorn AD. Compounds obtained from Sida acuta with the potential to induce quinone reductase and to inhibit 7, 12-dimehyl benz[a]anthracene-induced preneoplastic lesions in a mouse mammary organ culture model. Arch. Pharmacol. Res. 26, 2003, 585-590.
  9. [9]. Palaksha, MN., Ravishankar K. Phytochemical screening and evaluation of in vitro Antibacterial and Anthelmintic activities of Sida acuta leaf extracts. J Chemical and Pharmaceutical Research.4(11), 2012, 4757-4761.
  10. [10]. Damintoti Karou, Aly Savadogo, Antonella Canini, Saydou Yameogo, Carla Montesano, Jacques Simpore, Vittorio Colizzi, Alfard S., Traore. Antibacterial activity of alkaloids from Sida acuta. African Journal of Biotechnology. 4(12), 2005, 1452-1457.
  11. [11]. Sanganuwan, Alhaji Sanganuwan, Gulumbe, Mohammed Lawal. Evaluation of Sida acuta subspecie acuta leaf/flower combination for antimicrobial activity and phytochemical constituents. African Journal of Clinical and Experimental Microbiology. 7(2), 2006, 83-88.
  12. [12]. Marimuthu Govindarajan. Larvicidal and repellent activities of Sida acuta Burm.F.(Family: Malvaceae) against three important vector mosquitoes. Asian Pacific of Tropical Medicine.2010, 691-695.
  13. [13]. Akilandeswari, S., Senthamarai, R., Valarmathi, R., Shanthi, S., Prema, S. Screening of Gastric Antiulcer Activity of Sida acuta. International Journal of Pharm Tech Research. 2(2), 2010, 1644-1648.
  14. [14]. Adeniyi, SA., Orjiekwe, CL., Ehiagbonare, JE., Arimah BD. Preliminary phytochemical analysis and insecticidal activity of ethanolic extract of four tropical plants (Vernonia amygdalina, Sida acuta, Osimum gratissimum and Telfaria occidentalis) against beans weevil (Acanthscelides obtectus).Int. J. Phys. Sci.5(6),2010, 753-762.
  15. [15]. Okwuosa, CN., Azubike, NC., Nebo II. Evaluation of the Anti-Hyperglycemic Activity of the crude Leaf Extracts of Sida acuta in Normal and Diabetic Rabbits. Indian Journal of Novel Drug delivery. 3(3), 2011, 206-213.
  16. [16]. Sharma, R., Sharma, D., Kumar S. Antipyretic efficacy of Various Extract of Sida acuta leaves. Research Journal of Pharmaceutical, Biological and Chemical. 3(2), 2012, 515-518.
  17. [17]. Palaksha, MN., Ravishankar K. Phytochemical screening and evaluation of in vitro Antibacterial and Anthelmintic activities of Sida acuta leaf extracts. J Chemical and Pharmaceutical Research. 4(11), 2012, 4757-4761.
  18. [18]. Entaz Bahar, Joushan Ara, Mahbubul Alam, Bashutosh Nath, Unmesh Bhowmik, Nazmunnahar Runi. In-vitro Antioxidant and Thrombolytic activity of Methanol extract of Sida acuta. Journal of pharmacognosy and phytochemistry. 2(2), 2013, 125-133.
  19. [19]. Enemor, VHA., Okoye, VN., Awoke, UL. Effects of Ethanol Extract of Sida acuta Leaves on Some Organ Function Parameters and Physiologically Important Electrolytes in Normal Wister Albino Rats. American Journal of Drug Discovery and Development.2013:1-6
  20. [20]. Palaksha, MN., Ravishankar. K., Girja Sastry, V. Evaluation of Diuretic and Anti-Urolithiatic Activities of Ethanolic Leaf Extract of Sida acuta. American Journal of Pharmtech Research. 5(3), 2015, 197-207.
  21. [21]. Obioma Benedeth Eze, Okwesili Fred Nwodo. In-vitro stability and aggregatory effect of ethanol extract leaves of Sida acuta Burm F.on human erythrocyte. Journal of Experimental and Integrative Medicine. 6(3), 2016, 134-138.
  22. [22]. Obioma Benedeth Eze, Okwesili Fred Nwodo, Victor Nwadiogo Ogugua, Parker Elijah Joshua. Effect of ethanol extract of Sida acuta Burm F.leaves on egg albumin-induced inflammation. Journal of Experimental and Integrative Medicine. 6 (2),2016, 82-87.
  23. [23]. Kemi Feyisayo Akinwunmi, Abiodum Akeem Ajiboye ,Omolara Olajumoke Ojo. Evaluation of α – Amylase Inhibitory Potentials of Sida acuta, Tithonia diversifolia and Chromolaena odorate Leaf Extracts. Journal of Advances in Biology and Biotechnology. 14(4), 2017, 1-9.
  24. [24]. Sridevi, CD., Latha, PG., Ancy, P., Suja, SR., Shymal, S., Shine, VJ., Sini, S., Anuja, GI., Rajasekharan S. Hepatoprotective studies on Sida acuta Burm.f. Journal of Ethnopharmacology. 124(2), 2009, 171-175.
  25. [25]. Vimala, T., Gopalakrishnan, S. Inhibitory effect of the root of sida acuta Burm.f. on calcium oxalate crystal growth. The Journal of Research and Education in Indian Medicine. XVII (1), 2012, 21-26.
  26. [26]. Umoren, SA., Eduok, UM., Solomon, MM., Udoh, AP. Corrrosion inhibition by leaves and stem extract of Sida acuta for mild steel in 1 M H2SO4 solutions investigated by chemical and spectroscopic techniques. Arabian Journal of Chemistry. 9, 2016, S209-S224.
  27. [27]. Benzouzi, JT., Prado, R., Menan, H., Valentin, A., Roumestan, C., Mallie, M., Pelissier, Y., Blache, Y. Studies on medicinal plants of Ivory Coast: investigation of Sida acuta for in vitro antiplasmodial activities and identification of an active constituent. Phytomedicine. 11, 2004, 338-341.
  28. [28]. Oboh, IE., Onwukaeme, DN. Analgesic, anti-inflammatory and anti-ulcer activities of Sida acuta in mice and rat. Nig. J. Nat. Prod and Med. 09, 2005, 19-21.
  29. [29]. Otero, R., Nunez, V., Barona, J., Fonnegra, R., Jimenez, SL., Osoria, RG., Saldarriaga, M., Diaz, A. Snakebites and ethnobotony in the northwest region of Colombia. Part III: Neutralization of the haemorrhagic effect of Bothrops atrox venom. J Ethnopharmacol. 73, 2000, 233-44.
  30. [30]. Damintoti Karou, Mamoudou H. Dicko, Souleymane Sanon, Jacques Simpore, Alfard. S. Traore.Antimalarial activity of Sida acuta Burm.f (Malvacea) and Pterocarpus erinaceus Poir.(Fabaceae). Journal of Ethnopharmacology. 89, 2003, 291-294.
  31. [31]. Malairajan, P., Geetha Gopalakrishnan, S., Narasimhan, Jessi Kala Veni K. Antiulcer Activity of Sida acuta Burm. Natural Product Sciences. 12(3), 2006, 150-152.
  32. [32]. Akilandeswari, S., Senthamarai, R., Valarmathi, R., Prema, S. Wound Healing Activity of Sida acuta in Rats. International Journal of Pharm Tech Research. 2(1), 2010, 585-587.
  33. [33]. Pieme, CA., Penlap, VN., Ngogang, J., Costache M. In vitro cytotoxicity and antioxidant activities of five medicinal plants of Malvaceae family from Cameroon.Enviornmental Toxicology and Pharmacology. 29,2010, 223-228.
  34. [34]. Kannan, RR., Gnana, S. Vincent P. Cynoden dactylon and Sida acuta extracts impact on the function of the cardiovascular system in zebra fish embryos. J Biomed Res. 26(2), 2012, 90-97.
  35. [35]. Jindal Alka, Kumar Padma, Jain Chitra. Antifungal activity of Flavonoids of Sida acuta Burm f. against Candida albicans. International Journal of Drug Development and Research. 4(3), 2012, 92-96.
  36. [36]. Mahesh Thondawada, Shashank Mulukuta, Kalidhini Rama Satyanarayana Raju, Dhanabal, SP., Ashish Devidas Wadhwani. In vitro and In vivo Evaluation of Sida acuta Burm.f.(Malvaceae) its Anti-oxidant and Anti-Cancer Activity. Der PharmaChemica. 8(19), 2016, 396-402.
  37. [37]. Harborne JB, Phytochemical Analysis - A guide to modern techniques of plant analysis, 1998; Chapman & Hall, London.
  38. [38]. Rana MG. et al., In vitro antioxidant and free radical scavenging studies of alcoholic extract of medicago sativa L. 55(1) 1996, 15-22.
  39. [39]. Navnath et al., Free radical scavenging, reducing power and biochemical composition of Porphyra species. 1(2), 2010, 60-73.
  40. [40]. Prieto et al., In vitro free radical scavenging studies of methanolic extract of the leaves of mimusposelengi L. 6(2),1999, 197-202.