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May 1, 2021
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May 1, 2021
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Abstract

Aims and Objectives


To understand the influence of melatonin on body weight, plasma lipid, leptin and plasma insulin levels.


Materials and Methods


A total of 36 laboratory bred male Sprague dawley rats were used for the experiment. All the baseline parameters like body weight, lipid levels and plasma leptin and insulin levels were measured. The rats were fed with high fat diet starting from day 0 till 6 weeks. These rats were divided into two groups of 18 each. Group 1 (Control) received normal saline and Group 2 (Test) received 0.4mcg/ml of melatonin via oral gavage tube daily for six weeks. After six weeks, the parameters like Total body weight, lipid profile, plasma leptin and insulin levels were again observed. The statistical analysis of data was done using students‘t’ test, one way or two way ANOVA followed by post hoc bonferroni multiple comparison tests using SPSS. P value less than 0.05 was considered to be significant.


Results


The average weight gain in control group fed with fat rich diet was increased by 6% but the melatonin supplementation suppressed this increase to only 1.03%. Further, melatonin supplementation also suppressed the rise in total cholesterol, LDL, triglycerides and HDL cholesterol significantly (P<0.05). Melatonin administration not only suppressed the rise but also decreased the plasma leptin and insulin levels by 32% & 18.9% respectively after six weeks.


Conclusion


Daily supplementation of melatonin for six weeks in Sprague dawley rats fed with high fat diet suppresses the rise in body weight, plasma lipid levels and also decreases leptin and insulin levels significantly.

Keywords

SD Rats Cholesterol Lipids Fats Circadian rhythm Metabolic syndrome

Article Details

How to Cite
J Lalithaa Aparna, Satyajit Patil, S Ramamoorthy, & Rohit Dixit. (2021). Effect of melatonin on body weight, plasma lipid, leptin and insulin levels in high fat diet fed rats. International Journal of Research in Pharmacology & Pharmacotherapeutics, 6(3), 333-338. https://doi.org/10.61096/ijrpp.v6.iss3.2017.333-338
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