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Apr 22, 2021
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Apr 22, 2021
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Abstract

It is generally accepted that proteolytic enzymes are involved in the catabolic aspect of normal tissue remodeling and that altered activity of these enzymes is responsible for cartilage destruction and bone erosion associated with degenerative disorders such as rheumatoid arthritis (RA). In addition, reactive oxygen free radical species (ROS) have also been implicated in the pathogenesis of degenerative joint diseases. Hence the development of novel therapeutic strategies against rheumatoid arthritis should include both protease inhibitory and free radical scavenging elements. In the present study an in vitro evaluation of the free radical scavenging and protease inhibitory activities of the medicinal plant Datura stramonium L. has been attempted. Free radical scavenging activity was evaluated using the 1, 1-diphenyl, 2-picrylhydrazyl free radical (DPPH), 2, 2’-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl radical scavenging assays. The protease inhibitory activity was evaluated using the serine protease trypsin and the substrate BAEE (N-benzoyl-L-arginine ethyl ester).The plant extract showed significant radical scavenging activity with a percentage of inhibition of 51.8%, 78.7% and 67.7% for DPPH, ABTS and hydroxyl radical scavenging assays respectively. The plant also exhibited an inhibitory activity against protease with a percentage of inhibition of 58.30%. Results suggest that the plant extract possesses active principles capable of scavenging free radicals and inhibiting proteases that could be of therapeutic value in the management of rheumatoid arthritis.

Keywords

Datura stramonium L. Rheumatoid arthritis Free radical protease

Article Details

How to Cite
Punya Premrajan, & E. Jayadevi Variyar. (2021). Free radical scavenging and protease inhibitory activities of Datura stramonium L. International Journal of Research in Pharmacology & Pharmacotherapeutics, 4(3), 334-340. https://doi.org/10.61096/ijrpp.v4.iss3.2015.334-340
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