Article Sidebar

Submitted
Apr 21, 2021
Published
Apr 21, 2021
Download
PDF
Statistic
Read Counter : 47 Download : 24

Main Article Content

Abstract

Aim of the study: Elaeocarpus ganitrus (Family: Elaeocarpaceae), has shown benefit in the treatment of depression, convulsions and asthma. This study was undertaken to evaluate the antiparkinson effect of E.ganitrus.


Materials and methods: Parkinson’s disease was induced by administering MPTP (2 doses, each dose 20 mg/kg at 2 hr. interval, i.p. daily x 1week).The mice of either sex were divided into 06 groups (n =12).1st group mice were given 0.5% Carboxy methyl cellulose (orally), 2nd group were administered MPTP (2 doses, each dose 20 mg/kg at 2 hr. interval, i.p.). Whereas 3rd, 4th and 5th groups - were administered with E.ganitrus (100, 200, and 400 mg/kg/day,orally), respectively, along with MPTP. Group 6- received Levodopa (30mg/kg, i.p,) along with MPTP. Retention time in Rota rod test and latency period in catalepsy bar test was measured on day 0 and day 7. Assessment of oxidative stress was done by malondialdehyde (MDA) and reduced glutathione (GSH) measurement. One way ANOVA followed by post-hoc Tukey test, with p<0.05 considered statistical significant.


Results: E.ganitrus (200 and 400 mg/kg, p.o.) pretreated groups significantly increased the retention time in Rota rod test (p <0.001) and significantly decreased the latency period in catalepsy bar test (p <0.001) when compared to MPTP treated group alone. E.ganitrus (200 and 400 mg/kg, p.o.) pretreated groups showed significant antioxidative effect by decrease in brain MDA levels (p <0.001) and significant increase in brain GSH levels (p <0.001).


Conclusions: In our study, pretreatment with E. ganitrus showed antioxidative activity and increase in retention time in Rota rod test and decrease in latency period in Catalepsy bar test. The anti-oxidative property might be responsible for the changes in behavioral test parameters in the MPTP induced Parkinson’s disease in mice.

Keywords

E.ganitrus antioxidant MPTP MDA GSH

Article Details

How to Cite
Harish .G. Bagewadi, Naresh Khanna, Chetan .S. Bhangale, & Proteesh Rana. (2021). Beneficial effect of Elaeocarpus ganitrus on MPTP induced parkinson’s disease in mice. International Journal of Research in Pharmacology & Pharmacotherapeutics, 4(2), 187-193. https://doi.org/10.61096/ijrpp.v4.iss2.2015.187-193
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX

References

  1. [1]. Ates DA, Erdogrul OT. Antimicrobial activities of various medicinal and commercial plant extracts. Turk J Biol 2003;27:157-62.
  2. [2].Singh RK, Acharya SB, Bhattacharya SK. Pharmacological activity of Elaeocarpus Sphaericus. Phytother Res 2000; 14:36-39.
  3. [3].Singh RK, Bhattacharya SK, Acharya SB. Study of extract of Elaeocarpus ganitrus on in Vitro rat mast cells. Phyto Medicine 2000; 7:205-07.
  4. [4]. Katavic PL, Venables DA, Rali T, Carroll AR. Indolizidine alkaloids with delta opioid Receptor binding affinity from the leaves of Elaeocarpus fuscoides. J Nat Prod 2007; 69:1295-99.
  5. [5].Bhattacharya SK, Debnath PK, Pandey VB, Sanyal AK. Pharmacological investigations on Elaeocarpus ganitrus . Planta Medica 1975; 28:174-77.
  6. [6].Agid Y, Ruberg M, Javoy-Agid F, Hirsch E, Raisman-Vozari R, Vyas S, et al. Are Dopaminergic neurons selectively vulnerable to Parkinson's disease? Adv Neurol 1993; 60:148-164.
  7. [7].Prasad KN, Cole WC, Kumar B. Multiple antioxidants in the prevention and treatment of Parkinson's disease. J Am Coll Nutr 1999; 18:413-423.
  8. [8]. Kakalij RM, Alla CP, Kshirsagar RP, Kumar BH, Mutha SS, Diwan PV. Ameliorative Effect of Elaeocarpus ganitrus on gentamicin-induced nephrotoxicity in rats. Indian J Pharmacol 2014; 46:298-302.
  9. [9].Bagewadi HG, Afzal Khan AK. Evaluation of anti-parkinsonian activity of Elaeocarpus ganitrus on haloperidol induced Parkinson’s disease in mice. Int J Basic Clin Pharmacol 2015; 4:102-6.
  10. [10].Dunham NW, Miya TS. A note on a simple apparatus for detecting neurological deficit in ratsand mice. J Am Pharm.Assoc 1955; 46:208-209.
  11. [11].Hoffman DC, Donovan H. Catalepsy as a rodent model for detecting antipsychotic drugs with extrapyramidal side effect liability. Psychopharmacology 1995; 120:128-133.
  12. [12].Ohkawa H, Ohishi N, Yaki K. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95:351-358.
  13. [13].Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-77.
  14. [14]. Jenner, P. Preclinical evidence for neuroprotection with monoamine oxidase-B inhibitors in Parkinson's disease. Neurology 2004; 63:S13-S22.
  15. [15].Yuan H, Zheng JC, Liu P, Zhang SF, Xu JY, Bai LM. Pathogenesis of Parkinson's disease:oxidative stress, environmental impact factors and inflammatory processes. Neurosci Bull 2007; 23:125-130.
  16. [16]. Slivka A, Cohen G. Hydroxyl radical attack on dopamine. J Biol Chem 1985; 260:15466-15472.
  17. [17].Sriram K, Pai KS, Boyd MR, Ravindranath V. Evidence for generation of oxidative stressin brain by MPTP: in vitro and in vivo studies in mice. Brain Res 1997; 749:44-52.
  18. [18].Nair V, Arjuman A, Gopalakrishna HN, Nandini M.Effect of Withania somnifera root extract on haloperidol-induced catalepsy in albino mice. Phytother Res 2008; 22:243-246.
  19. [19].Mohanasundari M, Srinivasan MS, Sethupathy S, Sabesan M. Enhanced neuroprotective effect by combination of bromocriptine and Hypericum perforatum extract against MPTP-induced neurotoxicity in mice. J Neurol Sci 2006; 249:140-144.
  20. [20]. Polydoro M, Schroder N, Lima MN, Caldana F, Laranja DC, Bromberg E, et al. Haloperidol and clozapine induced oxidative stress in the rat brain. Pharmacology Biochem Behav 2004; 78:751-756.
  21. [21].Aubin N, Curet O, Deffois A, Carter C. Aspirin and salicylate protect against MPTP- induced dopamine depletion in mice. J Neurochem 1998; 71:1635-1642.
  22. [22].Teismann P et al.COX-2 and Neurodegeneration in Parkinson’s disease. Ann N Y Acad Sci 2003;991:272-277.
  23. [23].Singh RK, Pandey BL. Anti-inflammatory activity of Elaeocarpus sphaericus fruit extract in rats. J Med Arom Plant Sci1999;21:1030-32.
  24. [24].Singh B, Chopra A, Ishar MP, Sharma A, Raj T. Pharmacognostic and antifungal investigations of Elaeocarpus ganitrus (Rudrakasha). Indian J Pharm Sci 2010;72:261-65.
  25. [25]. Lal C. Tabulated phytochemical reports. Phytochemistry 1975;14:2727-28.
  26. [26]. Johns SR, Lamberton JA, Suares H, Sioumis AA, Willing RI. The alkaloids of Elaeocarpus sphaericus. Australian journal of chemistry 1971; 24:1679-94.
  27. [27]. Ray AB, Chand L, Pandey VB. Rudrakine, a new alkaloid from Elaeocarpus ganitrus . Phytochemistry 1979; 18:700-01.
  28. [28]. Chand L, Dasgupta S, Chattopadhyay SK, Ray AB. Chemical investigation of some Elaeocarpus species. Planta Medica 1977; 32:197-99.
  29. [29]. M.Vishnu Vardhan Reddy, Manmohan Singhal. Evaluation of Anti-Parkinsonian Activity of seed extract of Nelumbo Nucifera. Int J Pharm Bio Sci 2014;5:469-485.
  30. [30].Harish G Bagewadi, Naveen Rathor. Effect of Aloe vera on animal models of Parkinson disease in mice. Int J Pharm Bio Sci 2014;5(3):549 – 559.
  31. [31]. Sathish Kumar T, Shanmugam S, Palvannan T, Bharathi Kumar VM. Evaluation of Antioxidant Properties of Elaeocarpus ganitrus Roxb. Iranian Journal of Pharmaceutical Research 2008; 7:211-15.