Main Article Content
Abstract
Aim
To study the antiobesity and anticoagulant activity of ethanol extract of Trigonella foenum-graecum L. (Fenugreek) in male wistar rats.
Methods
Trigonella foenum-graecum L. was first extracted with ethanol and subjected to phytochemical analysis and its anti-obesity activity was studied in male wistar rats by feeding with cafeteria diet for 42 days in diet induced model and by administering a single intra peritoneal injection of Triton X-100 in chemical induced model. In diet induced model, weight of the animals was measured once in a week and parameters like total cholesterol (TC), triglycerides (TG), LDL, VLDL, HDL levels, clotting time were measured on 43rd day. In chemical induced model, the above parameters were measured on 8th day.
Results and Discussion
Preliminary phytochemical analysis revealed the presence of saponins, flavonoids, phyosterols, alkaloids, cardiac glycosides, coumarin glycosides, proteins and tannins & phenolic compounds. Significant increase in body weight, TC, TG, LDL, VLDL levels and reduction in HDL levels and clotting time was seen in animals fed with cafeteria diet. Treatment with standard drug orlistat and fenugreek extract significantly suppressed the increase in body weight and normalized the lipid levels and prolonged the clotting time.
Conclusion
From the results, it could be concluded that the ethanol extract of Trigonella foenum-graecum L. had significant anti-obesity and anticoagulant activity by maintaining the normal levels of physical and biochemical parameters and by prolonging the clotting time.
Keywords
Article Details
References
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- [2] Caballero B. The global epidemic of obesity: An overview, Epidemiol Rev, 2007; 29:1-5.
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- [8] Akter M, Afroze M, Khatun A. Evaluation of analgesic, neuropharmacological and cytotoxic activity of Trigonella foenum-graecum Linn. Int. cur.r Pharm.j. 2011, 1(1): 6-11.
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- [13] Vishnu Priya K, Himabindu P, A.B.Raju, Evaluation of antiobesity activity Of Fucus vesiculosus. Indian Journal of Research in Homoeopathy. 2013; 7(3):126-32.
- [14] Bhavesh L,Synthesis and pharmacological screening o some analogue havingaryl/ heteroaryl nucleus having alkylaminohydroxypropoxy side chain”, thesisPhD.2012.Avaliablefrom: http://etheses. saurashtrauniversity.edu /id/eprint/787
- [15] Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Am Assoc Clin Chem.1972;18:499‑502.
- [16] Harsh Mohan, Environmental and Nutritional diseases. In: Text book of pathology,6th edition. New Delhi: Jaypee Brother’s Medical Publishers (P) Ltd; 2010:243-245.
- [17] Gurevich P T, Soumya P, Emilia W , Marek Los. Obesity: Pathophysiology and Clinical Management. Current Medicinal Chemistry. 2009, 16, 506-521.
References
[1] Pilch PF and Bergenhem N. Pharmacological targeting of adipocytes/fat metabolism for treatment of obesity and diabetes. Mol Pharmacol. 2006; 70(3):779-85.
[2] Caballero B. The global epidemic of obesity: An overview, Epidemiol Rev, 2007; 29:1-5.
[3] Data and statistics [http://who.int/research/en/],(2012)
[4] Drew B, Dixon A and Dixon J. Obesity management: update on orlistat, Vasc Health Risk Manag.2007; 3: 817-821.
[5] The Global Epidemic [http://iaso.org/iotf/obesity/obesity the globalepidemic/] (2012)]
[6] Alshatwi A A, Shafi G, Noorul Hasan T, Naveed Ahmed S, K Khalid Khoja K. Fenugreek Induced Apoptosis in Breast Cancer MCF-7 Cells Mediated Independently by Fas Receptor Change. Asian Pac J Cancer Prev. 2013;14 (10):5783-5788.
[7] Palaniswamy M, Pradeep B.V, Sathya .R, Angayarkanni.J. In Vitro Anti-plasmodial Activity of Trigonella foenum–graecum L. eCAM 2010;7(4)441–445.
[8] Akter M, Afroze M, Khatun A. Evaluation of analgesic, neuropharmacological and cytotoxic activity of Trigonella foenum-graecum Linn. Int. cur.r Pharm.j. 2011, 1(1): 6-11.
[9] Margaret A. Allman-Farinelli, Obesity and Venous Thrombosis: A Review. Seminars in thrombosis and hemostasis. 2011;37(8):903-907.
[10] Rosito GA, D’Agostino RB, Massaro J, et al. Association between obesity and a prothrombotic state: the Framingham Offspring Study. Thromb Haemost 2004;91(4):683–689.
[11] Raskin I, Ribnicky D, M Komarnytsky S, Ilic N, Poulev A, Borisjuk N, et al.,Plants and human health in the twenty-first century. Trends Biotechnol. 2002, 20, 522-531.
[12] Khandelwal K.R. Preliminary phytochemical screening. Practical Pharmacognosy Techniques and Experiments, 9th edition..Pune: Nirali Publications 2000. P.149-156.
[13] Vishnu Priya K, Himabindu P, A.B.Raju, Evaluation of antiobesity activity Of Fucus vesiculosus. Indian Journal of Research in Homoeopathy. 2013; 7(3):126-32.
[14] Bhavesh L,Synthesis and pharmacological screening o some analogue havingaryl/ heteroaryl nucleus having alkylaminohydroxypropoxy side chain”, thesisPhD.2012.Avaliablefrom: http://etheses. saurashtrauniversity.edu /id/eprint/787
[15] Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Am Assoc Clin Chem.1972;18:499‑502.
[16] Harsh Mohan, Environmental and Nutritional diseases. In: Text book of pathology,6th edition. New Delhi: Jaypee Brother’s Medical Publishers (P) Ltd; 2010:243-245.
[17] Gurevich P T, Soumya P, Emilia W , Marek Los. Obesity: Pathophysiology and Clinical Management. Current Medicinal Chemistry. 2009, 16, 506-521.