@article{S Sathya_A Puratchikody_1, title={Role of β-sitosterol enriched fraction from the methanolic extract of leaves of Mallotus philippensis (Lam.) Muell. Arg. on inflammatory cytokines}, volume={5}, url={https://ijrpp.com/ijrpp/article/view/411}, DOI={10.61096/ijrpp.v5.issSPL1.2016.1-8}, abstractNote={<p>The potent pro-inflammatory cytokines (IL-1β &amp; TNF-α) and anti-inflammatory cytokine (IL-10) were investigated<br>for its effect on β-sitosterol enriched fraction from the methanolic extract of leaves of Mallotus philippensis (Lam.)<br>Muell. Arg. The n-butanol part of the methanolic extract was eluted using mixtures of solvents of increasing<br>polarity. From the combined eluted fractions, three fractions such as F1, F2 and F3 were collected by column<br>chromatography using n-hexane: Chloroform (1:1) as a solvent and the compounds present in the fractions were<br>identified by GC-MS. Among the collected fractions, the F1 was found to be the β-sitosterol enriched fraction <br>(β-SEF). The maximum non-lethal dose of β-SEF was found to be 1000 mg/kg body weight using OECD 423<br>guidelines. The effect of β-SEF (100 mg/kg), almost 10 times less than the standard drug Diclofenac sodium <br>(10 mg/kg), and it was determined against inflammation by carrageenan induced hind paw edema method. β-SEF<br>produced a dose dependent decrease in pro-inflammatory cytokines like IL-1β and TNF-α and increase <br>anti-inflammatory cytokine IL-10 level in the inflamed paw. Treatment with Diclofenac sodium (10 mg/kg) and <br>β-SEF (30 and 100 mg/kg) significantly decreased the NF-B expression in inflamed rats which concludes the<br>reduction of inflammation was probably by decreasing the expression of NF-B.</p&gt;}, number={SPL1}, journal={International Journal of Research in Pharmacology & Pharmacotherapeutics}, author={S Sathya and A Puratchikody}, year={1}, month={1}, pages={1-8} }